Over-Expression of Masson Pine PmPT1 Gene in Transgenic Tobacco Confers Tolerance Enhancement to Pi Deficiency by Ameliorating P Level and the Antioxidants

  • Ting Zhang
  • Yi Hong
  • XiaoPeng WenEmail author
Original Paper


Previously, we cloned the full sequence of masson pine (Pinus massoniana) phosphate transporter gene (PmPT1) from a phosphorus (Pi) deficiency tolerant strain. To further verify whether PmPT1 presumably function in angiosperms, i.e. tobacco, as well as to generate the new germplasm with high tolerance to Pi deficiency, currently, this gene was transferred into tobacco (Nicotiana tabacum) through Agrobacterium-mediated method. PmPT1 chiefly expressed in the roots of the transgenic plants, and considerably promoted the expression of two endogenous phosphate transporter genes of tobacco (NtPT1 and NtPT2) irrespectively of Pi status. Under low Pi conditions, the total P contents of the roots and shoots increased by 33.3% and 25.5%, respectively in L7, and by 30.7% and 23.9%, respectively in L18 in comparison with those of the wild type (WT). Also, the inorganic phosphorus (Pi) content of whole plants in L7 and L18 increased by 42.9% and 42.3%, respectively compared to the WT. The dry weight, contents of chlorophyll, soluble sugar and soluble protein, as well as the activities of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) were significantly elevated, conversely the MDA accumulation was obviously decreased in transgenic lines compared to the WT. Therefore, PmPT1, a phosphate transporter gene of Pht1 family from masson pine might function in tobacco and two overexpressed-PmPT1 transgenic lines substantially enhanced the tolerance of the transgenic tobacco to low-P stress, which was at least chiefly ascribed to the improvement of P accumulation and oxidant alleviation.


PmPT1 Genetic transformation Tobacco Low-phosphorus stress Antioxidant 



The project was supported by grants from National Key R & D Plan, P. R. China (2017YFD060030304), the Provincial Fundation of Guizhou Province, P. R. China (2019-1014), as well as the Opening Foundation of Key Laboratory of Educational Ministry (2018-474).

Author Contributions

TZ, YH, XPW designed the experiments. TZ, YH performed the experiments. TZ, YH, XPW computed, analyzed data. TZ, YH, XPW wrote the paper. All authors read and approved the manuscript.

Compliance with Ethical Standards

Conflict of Interest

The authors declared that no conflicts of interest to this work. We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Institute for Forest Resources & Environment of Guizhou, Institute of Agro-Bioengineering/College of Life SciencesGuizhou UniversityGuiyangChina
  2. 2.Guizhou Provincial Key Laboratory for Rare Animal and Economic Insects of the Mountainous Region, College of Biology and Environmental EngineeringGuiyang UniversityGuiyangChina

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