Two Genes (ClS1 and ClF-box) Involved the Self-Incompatibility of ”Xiangshui” Lemon (Citrus limon (L.) Burm. f.)
- 552 Downloads
Gametophytic self-incompatibility (GSI) is an important factor affecting fruit development and is one of the important mechanisms of seedlessness in citrus. Although many studies have investigated the mechanisms of SI, this process remains unclear in “Xiangshui” lemon (C. limon). In this study, we cloned one S-RNase homologous gene and one SLF homologous gene from the “Xiangshui” lemon, designated as ClS1 (pistil-specific expression) and ClF-box (pollen-specific expression), which contain open reading frames (ORFs) of 534 and 810 bp, respectively. The expression pattern of these two genes was analyzed by quantitative real-time polymerase chain reaction, and the results showed that the expression pattern of these two genes was significantly upregulated after self-pollination compared to that after cross-pollination. Through pollination in transgenic tobacco, we found that the pollen tubes grew slowly and became twisted after cross-pollination of ClS1♀ and ClF-box ♂ transgenic tobaccos, but the pollen tubes of control, self-pollination ClS1 and cross-pollination ClS1♂, and ClF-box ♀ grew normally. Furthermore, significantly fewer pollen tubes of ClS1♀ and ClF-box ♂ entered the ovules than that in other combinations, and the seed number of cross-pollination ClS1♀ and ClF-box ♂ transgenic tobaccos was significantly reduced. These results indicated that the ClS1 and ClF-box genes contribute to the self-incompatibility of the “Xiangshui” lemon.
KeywordsLemon Self-incompatibility S-RNase SLF Genetic transformation
This research was supported by the National Natural Science Foundation of China (31460508), Guangxi Natural Science Foundation under Grant No. 2018GXNSFAA294004 and the Innovation Team of the Guangxi Citrus Industry Project.
- Anderson MA, Cornish EC, Mau S-L, Williams EG, Hoggart R, Atkinson A, Bonig I, Grego B, Simpson R, Roche PJ, Haley JD, Penschow JD, Niall HD, Tregear GW, Coghlan JP, Crawford RJ, Clarke AE (1986) Cloning of cDNA for a stylar glycoprotein associated with expression of self-incompatibility in Nicotiana alata. Nature 321:38–44CrossRefGoogle Scholar
- Kao TH, McCubbin AG (1997) Molecular and biochemical bases of gametophytic self-incompatibility in Solanaceae. Plant Physiol Biochem 35:171–176Google Scholar
- Li TZ, Long SS, Li MF, Bai SL, Meng D (2011) Advances in research of the self-incompatibility genotypes (S-genotypes) in apple. Sci Agric Sin 44:1173–1183Google Scholar
- Luo W, Yang L (2006) Study on the optimization of transformation conditions for Agrohacterium tumefaciens. Biotechnology 16:41–43Google Scholar
- Ngo BX, Wakana A, Kim JH, Mori T, Sakai K (2010) Estimation of self-incompatibility Sgenotypes of citrus cultivars and plants based on controlled pollination with restricted number of pollen grains. J Fac Agric Kyushu Univ 55:67–72Google Scholar
- Qiao H, Wang H, Zhao L, Zhou J, Huang J, Zhang Y, Xue Y (2004a) The F-box protein AhSLF-S2 physically interacts with S-RNases that may be inhibited by the ubiquitin/26S proteasome pathway of protein degradation during compatible pollination in Antirrhinum. Plant Cell 16:582–595CrossRefPubMedPubMedCentralGoogle Scholar
- Shi GJ, Hou XL (2004) Measurement of self-incompatible by fluoroscope observation in non-heading Chinese cabbage. J Wuhan Bot Res 22:197–200Google Scholar
- Tao ST, Zhang SL, Chen DX, Wei BY (2004) Study on characteristics of in situ pollen germination and tube growth of Prunus mume. J Fruit Sci 21:338–340Google Scholar
- Ushijima K, Sassa H, Dandekar AM, Gradziel TM, Tao R, Hirano H (2003) Structural and transcriptional analysis of the self-incompatibility locus of almond: identification of a pollen-expressed F-box gene with haplotype-specific polymorphism. Plant Cell 15:771–781CrossRefPubMedPubMedCentralGoogle Scholar
- Wang HY, Xue YB (2005) Sub cellular localization of the S locus F-box protein AhSLF-S2 in pollen and pollen tubes of self-incompatible Antirrhinua. Plant Biol 47:76–83Google Scholar
- Wang XQ, Shen X, He YM, Ren TN, Wu WT, Xi T (2011) An optimized freeze-thaw method for transformation of Agrobacterium tumefaciens EHA105 and LBA4404. Pharm Biotechnol 18:382–386Google Scholar