Plant Molecular Biology Reporter

, Volume 37, Issue 1–2, pp 50–62 | Cite as

Two Genes (ClS1 and ClF-box) Involved the Self-Incompatibility of ”Xiangshui” Lemon (Citrus limon (L.) Burm. f.)

  • Wei Lin
  • Shuwei Zhang
  • Feng Ding
  • Xinhua HeEmail author
  • Cong Luo
  • Guixiang Huang
  • Minhphu Do
  • Qing Wang
  • Zaiyun Yang
  • Ling Su
  • Guibing Hu
Original Paper


Gametophytic self-incompatibility (GSI) is an important factor affecting fruit development and is one of the important mechanisms of seedlessness in citrus. Although many studies have investigated the mechanisms of SI, this process remains unclear in “Xiangshui” lemon (C. limon). In this study, we cloned one S-RNase homologous gene and one SLF homologous gene from the “Xiangshui” lemon, designated as ClS1 (pistil-specific expression) and ClF-box (pollen-specific expression), which contain open reading frames (ORFs) of 534 and 810 bp, respectively. The expression pattern of these two genes was analyzed by quantitative real-time polymerase chain reaction, and the results showed that the expression pattern of these two genes was significantly upregulated after self-pollination compared to that after cross-pollination. Through pollination in transgenic tobacco, we found that the pollen tubes grew slowly and became twisted after cross-pollination of ClS1♀ and ClF-box ♂ transgenic tobaccos, but the pollen tubes of control, self-pollination ClS1 and cross-pollination ClS1♂, and ClF-box ♀ grew normally. Furthermore, significantly fewer pollen tubes of ClS1♀ and ClF-box ♂ entered the ovules than that in other combinations, and the seed number of cross-pollination ClS1♀ and ClF-box ♂ transgenic tobaccos was significantly reduced. These results indicated that the ClS1 and ClF-box genes contribute to the self-incompatibility of the “Xiangshui” lemon.


Lemon Self-incompatibility S-RNase SLF Genetic transformation 


Financial Support

This research was supported by the National Natural Science Foundation of China (31460508), Guangxi Natural Science Foundation under Grant No. 2018GXNSFAA294004 and the Innovation Team of the Guangxi Citrus Industry Project.

Supplementary material

11105_2019_1135_MOESM1_ESM.docx (332 kb)
Supplementary Figure 1 (DOCX 331 kb)
11105_2019_1135_MOESM2_ESM.docx (27 kb)
Supplementary Figure 2 (DOCX 26 kb)
11105_2019_1135_MOESM3_ESM.docx (14 kb)
Supplementary Table 1 (DOCX 13 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Wei Lin
    • 1
  • Shuwei Zhang
    • 2
  • Feng Ding
    • 1
    • 2
  • Xinhua He
    • 1
    Email author
  • Cong Luo
    • 1
  • Guixiang Huang
    • 1
  • Minhphu Do
    • 1
  • Qing Wang
    • 1
  • Zaiyun Yang
    • 1
  • Ling Su
    • 1
  • Guibing Hu
    • 3
  1. 1.College of Agriculture, State Key Laboratory for Conservation and Utilization of Subtropical Agro-BioresourcesGuangxi UniversityNanningPeople’s Republic of China
  2. 2.Horticultural Research InstituteGuangxi Academy of Agricultural SciencesNanningPeople’s Republic of China
  3. 3.State Laboratory for Conservation and Utilization of Subtropical Agro-BioresourcesSouth China Agricultural UniversityGuangzhouChina

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