Plant Molecular Biology Reporter

, Volume 37, Issue 1–2, pp 1–13 | Cite as

External Supplement of Impulsive Micromanager Trichoderma Helps in Combating CO2 Stress in Rice Grown Under FACE

  • Aradhana MishraEmail author
  • Satyendra Pratap Singh
  • Sahil Mahfooz
  • Richa Shukla
  • Nishtha Mishra
  • Shipra Pandey
  • Sanjay Dwivedi
  • Vivek Pandey
  • Pramod Arvind Shirke
  • Chandra Shekhar Nautiyal
Original Paper


The present study aims to explore the alternative way to improve the quality and productivity of rice grown under CO2 stress through an external supplement of Trichoderma as a biofertilizer (BF). The impact of BF-treated rice under elevated CO2 (eCO2) was examined by different plant growth parameters, physiological observations, scanning electron microscopy, microbial community profiling and expression levels of stress-related genes. The effect of eCO2 on percent change in yielding attributes of rice (Heena and Kiran) was found higher in control, whereas it was reduced in the presence of BF. Photosynthetic rate, stomatal conductance and transpiration rate were higher in BF-treated rice under eCO2 condition. SEM analysis of BF-treated roots exhibits an increase in the number of metaxylem along with its diameter with thicker and rigid sclerenchymatous cells. Expression analysis of stress-related genes showed an increase in their mRNA transcripts under eCO2 condition. A significant change in the microbial community was found in the rhizospheric region of Heena treated with BF under eCO2. The current study demonstrates the potential of BF in ameliorating the stress generated as a result of CO2 enrichment.


Elevated CO2 FACE Microbial diversity SEM Stress genes T. reesei 



The authors are grateful to Dr. P. N. Saxena (CSIR-Indian Institute of Toxicology Research, Lucknow) for SEM micrograph imaging.

Funding Information

This work is supported by funding from Science and Engineering Research Board, New Delhi (GAP3349), and Council of Scientific and Industrial Research (PSC0112).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no competing interest

Supplementary material

11105_2018_1133_MOESM1_ESM.doc (40 kb)
ESM 1 (DOC 41 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Aradhana Mishra
    • 1
    Email author
  • Satyendra Pratap Singh
    • 1
  • Sahil Mahfooz
    • 1
  • Richa Shukla
    • 1
  • Nishtha Mishra
    • 1
  • Shipra Pandey
    • 1
  • Sanjay Dwivedi
    • 2
  • Vivek Pandey
    • 3
  • Pramod Arvind Shirke
    • 3
  • Chandra Shekhar Nautiyal
    • 1
  1. 1.Division of Plant Microbe InteractionsCSIR-National Botanical Research InstituteLucknowIndia
  2. 2.Division of Plant Ecology and Environmental SciencesCSIR-National Botanical Research InstituteLucknowIndia
  3. 3.Plant Physiology LabCSIR-National Botanical Research InstituteLucknowIndia

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