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Characterization of Eukaryotic Release Factor 3 (eRF3) Translation Termination Factor in Plants

  • Andor Auber
  • Tünde Nyikó
  • Zsuzsanna Mérai
  • Dániel Silhavy
Original Paper
  • 24 Downloads

Abstract

Eukaryotic translation termination is mediated by two conserved interacting release factors, eRF1 and eRF3. eRF1 recognizes the stop codon and promotes the hydrolysis of the polypeptide chain, while eukaryotic eRF3 stimulates eRF1 release activity in the presence of GTP. It is widely believed that translation termination is highly conserved in eukaryotes. However, recent results that eRF1 is present in multiple, partially redundant copies in plants and that eRF1 expression is controlled by a complex, plant-specific autoregulatory circuit suggest that regulation of translation termination might be especially complex in plants. Surprisingly, very little is known about translation termination in plant, for instance, the eRF3 termination factor has not been analyzed in plants yet. Thus, we wanted to identify and characterize the eRF3 translation termination factor in plants. By combining a range of transient and transgenic assay here, we identified plant eRF3 and showed that it directly interacts with eRF1. In contrast to eRF1, plant eRF3 is not autoregulated, while eRF3 and eRF1 expressions are connected. We also demonstrated that eRF3 interacts with the core NMD factor, UPF1, and the expression of eRF3 is NMD regulated in certain plant species suggesting that in addition to the normal translation termination, eRF3 could be connected to plant nonsense-mediated decay (NMD). Finally, it appears that the plant termination factors are present in physiologically different concentrations, while eRF1 concentration limits the efficiency of both translation termination and NMD, eRF3 is present in non-limiting concentration.

Keywords

Translation termination complex eRF3 Cross-regulation NMD 

Abbreviations

eRF1

eukaryotic release factor 1

eRFR3

eukaryotic release factor 3

GTP

guanosine-5′-triphosphate

GTPase

guanosine triphosphatase

PABP

poly (A) binding protein

PAM2

PABP-interacting motif 2

NMD

nonsense-mediated decay

RT

translational readthrough

PTC

premature termination codon

EJC

exon-junction complex

ORF

open reading frame

uORF

upstream open reading frame

U1DN

dominant-negative version of UPF1 NMD factor

Notes

Funding Information

This work was supported by the Agricultural Ministry of Hungary, the Hungarian Scientific Research Fund (NKFIH OTKA K109835 and K116963). A. Auber is a graduate student of the ELTE “Classical and Molecular Genetics” PhD program. We are grateful for K. Riha for the smg7-1 line.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

11105_2018_1128_MOESM1_ESM.pdf (1.4 mb)
ESM 1 (PDF 1430 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of GeneticsAgricultural Biotechnology InstituteGödöllőHungary
  2. 2.Gregor Mendel InstituteAustrian Academy of SciencesViennaAustria

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