Plant Molecular Biology Reporter

, Volume 36, Issue 5–6, pp 822–831 | Cite as

Early Changes in S-Nitrosoproteome in Soybean Seedlings Under Flooding Stress

  • Akiko HashiguchiEmail author
  • Setsuko KomatsuEmail author
Original Paper


The shift from aerobic to anaerobic respiration is crucial for soybean response to flooding stress; however, the regulatory mechanism in action at the initial stage of flooding stress has not been fully elucidated. To identify this mechanism in soybean, proteomic analysis of S-nitrosylated proteins was performed with emphasis on nitric oxide (NO)-mediated regulation in soybean seedlings. Removal of NO by addition of 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) partially restored seedling growth. After 3, 9, and 24 h of flooding stress, the S-nitrosylation status of 364, 188, and 186 proteins was altered relative to the corresponding status before flooding, respectively. Abundance of S-nitrosylated forms of 2, 186, and 162 proteins differed between the untreated control and flooded soybean plants after 3, 9, and 24 h of flooding stress, respectively. After flooding for 3 h, development, stress, and glycolysis/fermentation categories were identified as the top categories including proteins for which abundance of S-nitrosylated forms increased. Visualization of changes in S-nitrosylation profile by pathway mapping indicated a characteristic pattern in glycolysis/fermentation. Western blot analysis confirmed that S-nitrosylated status of alcohol dehydrogenase increased with flooding. These results suggest that S-nitrosylation comprises rapid molecular processes that change the abundance of the active form of alcohol dehydrogenase.


Soybean Flooding stress S-nitrosylation Fermentation Alcohol dehydrogenase 



Post-translational modification


Nitric oxide


Glyceraldehyde-3-phosphate dehydrogenase


Alcohol dehydrogenase




Liquid chromatography


Mass spectrometry



We are grateful to Dr. Xin Wang for the technical support of this research.


This work was supported by JSPS KAKENHI Grant Number JP15H04445.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Ethical Statement

This article does not contain any studies with human or animal participants performed by any of the authors.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Faculty of MedicineUniversity of TsukubaTsukubaJapan
  2. 2.National Institute of Crop ScienceTsukubaJapan
  3. 3.Faculty of Environmental and Information SciencesFukui University of TechnologyFukuiJapan

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