Transcriptomic Profiling of Lathyrus sativus L. Metabolism of β-ODAP, a Neuroexcitatory Amino Acid Associated with Neurodegenerative Lower Limb Paralysis
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Grass pea (Lathyrus sativus L.) is a unique potential crop for marginal arid regions with untapped, exceptional biotic/abiotic stress tolerance, and high protein content seeds. High dietary intake of grass pea is associated with neurodegenerative lower limb paralysis, thought due to the compound β-N-oxalyl-L-α, β-diaminopropionic acid (β-ODAP). β-ODAP increases after seed germination and then decreases subsequently. To monitor changes in gene expression during seed germination, RNA sequencing was performed on seedlings at 2, 6, and 25 days after sowing. A total of 213,258 unigenes were detected, corresponding to 39,548 coding sequences. Extensive Gene Ontology and coexpression network analysis suggested that primary metabolism, particularly carbohydrates and sulfur, were correlated with β-ODAP content. Our results identified transcriptional profiles related to grass pea seedling development, and provide invaluable insight into mechanisms of β-ODAP accumulation and degradation. Defining the gene space is essential for intelligent genetic, biotechnological, and breeding efforts to reduce β-ODAP for human benefit.
Keywordsβ-ODAP Lathyrus sativus Transcriptome Gene expression Seedling
Quanle Xu, Peng Chen, and Hari Krishnan designed the study. Quanle Xu, Jason D. Gillman, Peng Chen, and Hari Krishnan wrote and edited the manuscript. Fengjuan Liu and Ruihong Qu performed qRT-PCR validation. Fengjuan Liu, Jason D. Gillman, Chunxiao Bi, and Xin Hu carried out bioinformatics analysis. All authors read and approved the final manuscript.
This study was supported by the National Natural Science Foundation (31401910), China Postdoctoral Science Foundation (2016M590975), Postdoctoral Science Foundation of Shaanxi province (2016BSHEDZZ119), and the Fundamental Research Funds for the Central Universities (2014YB040), P.R. China.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
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