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Plant Molecular Biology Reporter

, Volume 36, Issue 5–6, pp 776–789 | Cite as

CanTF, a Novel Transcription Factor in Pepper, Is Involved in Resistance to Phytophthora capsici as well as Abiotic Stresses

  • Yu-Mei He
  • De-Xu Luo
  • Abid Khan
  • Ke-Ke Liu
  • Mohamed Hamed Arisha
  • Huai-Xia Zhang
  • Guo-Xin Cheng
  • Xiao Ma
  • Zhen-Hui GongEmail author
Original Paper
  • 176 Downloads

Abstract

Stress is usually considered an important factor resulting in plant injury. In this study, we identified a novel transcription factor gene, CanTF (Capsicum annuum transcription factor IIB), and characterized its role in the response to biotic and abiotic stresses. The full-length CanTF cDNA consists of 1488 bp, with a 1281-nucleotide open reading frame (ORF), and encodes a protein containing 426 amino acids with a theoretical molecular weight of 46.84 kDa. Real-time quantitative PCR revealed that CanTF is a stress-induced gene, with increased expression levels under both biotic and abiotic stresses. The expression of CanTF in pepper organs, especially roots, was highly induced by inoculation with an avirulent strain of Phytophthora capsici. Additionally, the differential expression of CanTF was observed under abiotic stresses, i.e., earlier expression was detected after cold, drought, and SA treatments than after salt and H2O2 treatment, suggesting its role in responses to various abiotic stresses. Furthermore, the silencing of CanTF by virus-induced gene silencing reduced the expression of defense-related genes (CaPR1, CaDEF1, and CaSAR82) under P. capsici inoculation. POD and root activity levels were lower after gene silencing than in controls, demonstrating the positive regulatory effect of CanTF against P. capsici. These results suggested that CanTF is a stress-induced gene involved in strengthening the pepper defense against biotic and abiotic stresses.

Keywords

Pepper Transcription factor (TF) Phytophthora capsici Abiotic stress Virus-induced gene silencing 

Notes

Author Contributions

YMH, DXL, and ZHG conceived the research. YMH, KKL, HXZ, AK, GXC, and XM performed the research. YMH, HXZ, and MHA performed statistical analyses. YMH and AK wrote the paper. YMH, MHA, AK, and ZHG revised the paper. DXL and ZHG provided the materials and resources for the research. YMH, KKL, and ZHG performed the integrity of the work. All authors read and approved the final manuscript.

Funding information

This work was supported through funding from the National Key R&D Program of China (No. 2016YFD0101900), the National Natural Science Foundation of China (No. U1603102), and the Independent Innovation Fund Project of Agricultural Science and Technology in Jiangsu (NO.CX (17) 3040).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Yu-Mei He
    • 1
  • De-Xu Luo
    • 2
  • Abid Khan
    • 1
  • Ke-Ke Liu
    • 1
    • 3
  • Mohamed Hamed Arisha
    • 1
    • 4
  • Huai-Xia Zhang
    • 1
  • Guo-Xin Cheng
    • 1
  • Xiao Ma
    • 1
  • Zhen-Hui Gong
    • 1
    Email author
  1. 1.College of HorticultureNorthwest A&F UniversityYanglingPeople’s Republic of China
  2. 2.Xuhuai Region Huaiyin Institute of Agricultural SciencesHuaianPeople’s Republic of China
  3. 3.College of HorticultureHenan Agricultural UniversityZhengzhouChina
  4. 4.Department of Horticulture, Faculty of AgricultureZagazig UniversityZagazigEgypt

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