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Plant Molecular Biology Reporter

, Volume 36, Issue 5–6, pp 812–821 | Cite as

Identification of Genes Involved in Flavonoid Biosynthesis of Chinese Narcissus (Narcissus tazetta L. var. chinensis)

  • Guiqing Wang
  • Biyun Yang
  • Jiacheng Wu
  • Peng Luo
  • Muhammad Anwar
  • Andrew C. Allan
  • Kui Lin-Wang
  • Richard V. Espley
  • Lihui ZengEmail author
Original Research
  • 203 Downloads

Abstract

Chinese narcissus (Narcissus tazetta L. var. chinensis Roem.) is a popular flower in Asia. However, flower colors are limited with all cultivars having a white perianth and yellow corona. Previous studies have shown no anthocyanin accumulation in this species. The reason for an absence of anthocyanins remains unknown, with the flavonoid biosynthetic pathway in this flower recently being investigated. In this study, a transcriptomic approach combined with gene expression and biochemical analysis was used to predict and annotate genes in the flavonoid biosynthetic pathway. Results showed that the major flavonoid metabolites are flavonols and proanthocyanidins. Proanthocyanidin biosynthesis is exclusively catechin-based, with the gene encoding leucoanthocyanidin reductase (LAR) being well expressed. We identified 16 unigenes encoding key enzymes involved in flavonoid biosynthesis. Flavonol synthase (FLS) and dihydroflavonol reductase (DFR) appear to play important roles in regulating proanthocyanidin and flavonol levels, while anthocyanidin synthase (ANS) expression is not detected. Our results suggest that the absence of anthocyanins in Chinese narcissus is due to high expression of LAR and FLS, and a lack of ANS expression.

Keywords

Chinese narcissus Flavonoid metabolic pathway Structural genes Anthocyanin 

Notes

Funding Information

This work was supported by Fujian Natural Science Fund, China (No. 2016J01109) and Fujian Agriculture and Forestry University International Scientific and Technological Exchange and Cooperation Project, China (Kxb16013A).

Supplementary material

11105_2018_1119_MOESM1_ESM.docx (610 kb)
ESM 1 (DOCX 609 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of HorticultureFujian Agriculture and Forestry UniversityFuzhouPeople’s Republic of China
  2. 2.The New Zealand Institute for Plant & Food Research Ltd, (Plant and Food Research)Mt Albert Research CentreAucklandNew Zealand

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