Intronic Sequence Variations in a Gene with Peroxidase Domain Alter Bolting Time in Cabbage (Brassica oleracea var. capitata)
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Cabbage (Brassica oleracea var. capitata) is the most popular leafy vegetable; however, its quality as a vegetable depends on its growth stage. Premature bolting triggered by low temperatures leads to a reduction of yield and quality of cabbage. Late bolting is preferred by growers to increase market value, whereas early bolting plants are ideal for quality seed production. Herein, we reported a gene BolPrx.2 annotated as Q9FLC0 in the SwissPort, involved in bolting time variation in cabbage and designed molecular markers to characterize early- and late-bolting cabbage populations and lines. The BolPrx.2 gene encodes a peroxidase domain and has been identified as a candidate showed almost similar effect as the previously reported MADS-box domain-containing FLC genes for controlling bolting time. An insertion/deletion (InDel) variation in intron1 has been identified as a causal factor for variation between late- and early-bolting inbred lines. By using this InDel, we designed molecular markers for characterizing the bolting time variation and validated them with 141 F2 generation plants. These markers predicted about 84% of the variation within the population and commercial lines. Therefore, it could be a potential genetic tool to predict bolting time variation and support marker-assisted back crossing (MABC) programs for developing desired bolting types of cabbage cultivars.
KeywordsInDel markers Peroxidase domain Intron sequence Early- and late-bolting cabbages
The work presented here was carried out in collaboration among all authors. MA carried out the experiments and prepared the tables, figures, and manuscript draft. UKN collected primary data regarding genes and wrote, edited, and finalized the manuscript. JIP formulated the experimental concept and provided the plant materials. HTK conducted greenhouse experiments and took care of the plant populations. MKB performed in silico analyses and helped with cloning. ISN designed and participated in all the experiments and assisted in improving the technical sites for the project. All authors have read and approved the final manuscript.
This research was supported by the Golden Seed Project (Center for Horticultural Seed Development, No. 213007-05-2-SB110), the Ministry of Agriculture, the Food and Rural Affairs (MAFRA), the Ministry of Oceans and Fisheries (MOF), the Rural Development Administration (RDA), and Korea Forest Service (KFS), Korea.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
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