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CarHSFB2, a Class B Heat Shock Transcription Factor, Is Involved in Different Developmental Processes and Various Stress Responses in Chickpea (Cicer Arietinum L.)

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Abstract

Heat shock transcription factor (HSF) plays an essential role on the increased tolerance against heat stress by regulating the expression of heat-responsive genes. In this study, a HSF gene, CarHSFB2, was isolated and characterized in chickpea. CarHSFB2 was a nuclear protein with a predicted polypeptide of 267 amino acids and encoded by a single/low copy genes. Phylogenetic analysis showed that CarHSFB2 belonged to the class B HSFs. It had little or no any transcription activation activity due to lack of aromatic, hydrophobic, and acidic amino acid (AHA) motifs. CarHSFB2 showed different expression patterns among different developmental processes (leaf senescence, developing seed, and embryo of germinating seed). It was induced by the stress of heat, salt, wound and drought, and the treatment of H2O2, IAA, and GA3, respectively, while inhibited by 6-BA. However, the other stress and chemical treatments (cold, ABA, MeJA, Et, and SA) had no obvious effect on its expression. Overexpression of CarHSFB2 in Arabidopsis seedlings showed the increased tolerance to drought and heat stress. Additionally, stress-responsive genes, RD22, RD26, and RD29A, showed significantly higher expression levels in transgenic Arabidopsis seedlings than in the wild type (WT) under drought stress, whereas HsfA2, HsfB2a, and HsfA7a in transgenic Arabidopsis seedlings were markedly accumulated in transcript level than in the WT under heat stress. All these results indicate that CarHSFB2, a class B HSF, positively functions in different developmental processes and various stress responses, especially in positive response to heat and drought stresses, in chickpea.

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Abbreviations

6-BA:

N-6-benzyl-adenine

ABA:

Abscisic acid

AHA:

Aromatic, hydrophobic, and acidic amino acids

CTD:

C-terminal domain

CTR:

C-terminal region

DAF:

Days after flowering

DBD:

DNA-binding domain

Et:

Ethephon

EST:

Expressed sequence tag

GA3:

Gibberellin

GSP:

Gene-specific primer

IAA:

Indole-3-acetic acid

HAI:

Hours after the initial imbibition

HR-A/B:

Heptad pattern of hydrophobic residues

HSE:

Heat stress element

HSP:

Heat shock protein

HSF:

Heat shock transcription factor

Lp:

Lycopersicon peruvianum

MeJA:

Methyl jasmonate

NGSP:

Gene-specific primer for the nested PCR

NLS:

Nuclear localization signal

ORF:

Open reading frame

Os:

Oryza sativa

RACE:

Rapid amplification of cDNA end

RT-PCR:

Reverse transcription-polymerase chain reaction

SA:

Salicylic acid

UTR:

Untranslated region

WT:

Wild type

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Acknowledgments

We gratefully acknowledge the partial financial support from the projects supported by the National Natural Science Foundation of China (31160306, 30960201, 30860152, and 31160300) and the Xinjiang Science and Technology Department of China (200991254) for this research.

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Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China

    Hao Ma, Chuntao Wang, Bin Yang, Huiying Cheng, Cai Ren & Guanghang Qu

  2. Desert Research Institute in the Arid Region, |Xinjiang Agricultural University, Urumqi, 830052, China

    Hao Ma, Ze Wang, Abudoukeyumu Mijiti, Hua Zhang & Lin Ma

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  1. Hao Ma
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  2. Chuntao Wang
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  3. Bin Yang
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  9. Hua Zhang
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  10. Lin Ma
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Correspondence to Hao Ma.

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Ma, H., Wang, C., Yang, B. et al. CarHSFB2, a Class B Heat Shock Transcription Factor, Is Involved in Different Developmental Processes and Various Stress Responses in Chickpea (Cicer Arietinum L.). Plant Mol Biol Rep 34, 1–14 (2016). https://doi.org/10.1007/s11105-015-0892-8

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