The rhizosheath: a potential root trait helping plants to tolerate drought stress

  • Majid Basirat
  • Seyed Majid MousaviEmail author
  • Shirzad Abbaszadeh
  • Mohsen Ebrahimi
  • Mohsen Zarebanadkouki
Regular Article



Rhizosheath is known as a layer of adhering soil particle to the root surface. Despite several speculations, the positive function of rhizosheath in acquisition of water and nutrients from drying soil has not yet been experimentally proven. The objective of this study was to experimentally show whether an enhanced rhizosheath formation could help plants to better access water from drying soil.


Eight wheat cultivars were grown in a sandy-loam soil. When plants were 35 days old let dry soil to a water content at which evident wilting symptoms appeared on the plant leaves. During this drying cycle, soil water content and transpiration rate of plants were gravimetrically measured by weighing the plant pots. At the end of this drying cycle, the roots were excavated out of the soil and the rhizosheath formation was gravimetrically quantified by weighing the soil attached to the root system.


The results showed that plant cultivars with greater rhizosheath formation could sustain higher transpiration rates at dry condition (water content of 0.07 cm3 cm−3) while the plant cultivars with lower rhizosheath formation suffered from drought stress and reached their permanent wilting points at the same water content.


The findings of this study gathered evidence that under severe drought condition plant cultivars with an enhanced rhizosheath formation could better survive by sustaining their transpirational and nutritional demands.


Drought tolerant Mucilage Rhizosheath Rhizosphere Transpiration Wheat 



  1. Ahmed MA, Zarebanadkouki M, Meunier F, Javaux M, Kaestner A, Carminati A (2018) Root type matters: measurement of water uptake by seminal, crown, and lateral roots in maize. J Exp Bot 69(5):1199–1206. CrossRefPubMedPubMedCentralGoogle Scholar
  2. Benard P, Zarebandkouki M, Brax M, Kaltenbach R, Jerjen I, Marone F, Couradeau E, Felde V, Kaestner A, Carminati A (2019) Micro-hydrological niches in soils: how mucilage and EPS alter the biophysical properties of the rhizosphere and other biological hot spots. Vadose Zone J. CrossRefGoogle Scholar
  3. Bengough AG, Bransby MF, Hans J, McKenna SJ, Roberts TJ, Valentine TA (2006) Root responses to soil physical conditions; growth dynamics from field to cell. J Exp Bot 57:437–447. CrossRefGoogle Scholar
  4. Blum A (1996) Crop responses to drought and the interpretation of adaptation. Plant Growth Regul 20:135–140. CrossRefGoogle Scholar
  5. Bristow CE, Campbell GS, Wullstein LH, Neilson R (1985) Water-uptake and storage by rhizosheaths of Oryzopsis hymenoides – a numerical simulation. Physiol Plant 65:228–232. CrossRefGoogle Scholar
  6. Brown LK, George TS, Thompson JA, Wright G, Lyon J, Hubbard SF, White PJ (2012) What Are the Implications of Variation in Root Hair Length on P-Limited Yield in Barley (Hordeum vulgare L.)?. Annals of Botany 10: 319–28. CrossRefGoogle Scholar
  7. Brown LK, George TS, Neugebauer K, White PJ (2017) The Rhizosheath – a potential trait for future agricultural sustainability occurs in orders throughout the angiosperms. Plant Soil 418(1). CrossRefGoogle Scholar
  8. Carminati A, Vetterlein D (2013) Plasticity of Rhizosphere hydraulic properties as a key for efficient utilization of scarce resources. Ann Bot 112(2):277–290. CrossRefPubMedGoogle Scholar
  9. Carminati A, Schneider CL, Moradi AB, Zarebanadkouki M, Vetterlein D, Vogel HJ, Hildebrandt A, Weller U, Schüler L, Oswald SE (2011) How the rhizosphere may favor water availability to roots. Vadose Zone J 10:988–998. CrossRefGoogle Scholar
  10. Delhaize E, James RA, Ryan PR (2012) Aluminium tolerance of root hairs underlies genotypic differences in Rhizosheath size of wheat (Triticum Aestivum) grown on acid soil. New Phytol 195:609–619. CrossRefPubMedGoogle Scholar
  11. Delhaize E, Rathjen TM, Cavanagh CR (2015) The genetics of rhizosheath size in a multiparent mapping population of wheat. J Exp Bot 66:4527–4536. CrossRefPubMedPubMedCentralGoogle Scholar
  12. Fang Y, Xiong L (2015) General mechanisms of drought response and their application in drought resistance improvement in plants. Cell Mol Life Sci 72:673–689. CrossRefPubMedGoogle Scholar
  13. Gao Y, Yang Y, Ling W, Kong H, Zhu X (2011) Gradient distribution of root exudates and polycyclic aromatic hydrocarbons in rhizosphere soil. Soil Sci Soc Am J 75:1694–1703. CrossRefGoogle Scholar
  14. Gardner WR (1960) Dynamic aspects of water availability to plants. Soil Sci 89(2):63–73CrossRefGoogle Scholar
  15. George TS, Brown LK, Ramsay L, White PJ, Newton AC, Bengough AG, Russell J, Thomas WT (2014) Understanding the genetic control and physiological traits associated with rhizosheath production by barley (Hordeum Vulgare). New Phytol 203:195–205. CrossRefPubMedGoogle Scholar
  16. Ghezzehei TA, Albalasmeh AA (2015) Spatial distribution of rhizodeposits provides built-in water potential gradient in the rhizosphere. Ecol Model 298:53–63. CrossRefGoogle Scholar
  17. Guo HG, Wang WB, Luo XH, Wu XP (2015) Characteristics of rhizosphere and bulk soil microbial communities in rubber plantations in Hainan Island, China. J Trop For Sci 27(2):202–212Google Scholar
  18. Haling RE, Richardson AE, Culvenor RA, Lambers H, Simpson RJ (2010a) Root morphology, root-hair development and rhizosheath formation on perennial grass seedlings is influenced by soil acidity. Plant Soil 335(1):457–468. CrossRefGoogle Scholar
  19. Haling RE, Simpson RJ, Delhaize E, Hocking PJ, Richardson AE (2010b) Effect of lime on root growth, morphology and the rhizosheath of cereal seedlings growing in an acid soil. Plant Soil 327(1–2):199–212. CrossRefGoogle Scholar
  20. Haling RE, Brown LK, Bengough AG, Young IM, Hallett PD, White PJ, George TS (2013) Root hairs improve root penetration, root–soil contact, and phosphorus acquisition in soils of different strength. J Exp Bot 64(12):3711–3721. CrossRefGoogle Scholar
  21. Haling RE, Brown LK, Bengough AG, Valentine TA, White PG, Young IM, George TS (2014) Root hair length and rhizosheath mass depend on soil porosity, strength and water content in barley genotypes. Planta 239:643–651. CrossRefGoogle Scholar
  22. Hartnett DC, Wilson GW, Ott J, Setshogo M (2013) Variation in root system traits among african semi-arid savanna grasses: implications for drought tolerance. Austral Ecology 38(4):383–392. CrossRefGoogle Scholar
  23. Holz M, Zarebanadkouki M, Kuzyakov Y, Pausch J, Carminati A (2018) Root hairs increase rhizosphere extension and carbon input to soil. Ann Bot 121(1):61–69. CrossRefPubMedGoogle Scholar
  24. Leitner D, Meunier F, Bodner G, Javaux M, Schnepf S (2014) Impact of contrasted maize root traits at flowering on water stress tolerance – a simulation study. Filed Crop Research 165:125–137. CrossRefGoogle Scholar
  25. Liu TY, Ye N, Song T, Cao Y, Gao B, Zhang D, Zhu F, Chen M, Zhang Y, Xu W, Zhang J (2018) Rhizosheath formation and involvement in foxtail millet (setaria italica) root growth under drought stress. J Integr Plant Biol 61(4):449–462. CrossRefPubMedGoogle Scholar
  26. Lynch JP (2013) Steep, cheap and deep: an ideotype to optimize water and N acquisition by maize root systems. Ann Bot 112(2):347–357. CrossRefPubMedPubMedCentralGoogle Scholar
  27. McCully ME (1999) Roots in soil: unearthing the complexities of roots and their Rhizospheres. Annu Rev Plant Physiol Plant Mol Biol 50:695–718. CrossRefPubMedGoogle Scholar
  28. Motzo R, Giunta F (2007) The effect of breeding on the phenology of Italian durum Wheats: from landraces to modern cultivars. Agronomy 26:462–470. CrossRefGoogle Scholar
  29. Nambiar EKS (1976) The uptake of Zinc-65 by oats in relation to soil water content and root growth. Soil Research 14(1):67–74. CrossRefGoogle Scholar
  30. North GB, Nobel PS (1997) Drought-induced changes in soil contact and hydraulic conductivity for roots of opuntiaficus-indicawith and without rhizosheaths. Plant Soil 191: 249–258. 1023/A:1004213728734Google Scholar
  31. Oburger E, Jones DL (2018) Sampling root exudates – Mission impossible? Rhizosphere 6:116–133. CrossRefGoogle Scholar
  32. Othman AA, Amer WM, Fayez M, Hegazi NA (2004) Rhizosheath of Sinai desert plants is a potential repository for associative diazotrophs. Microbiol Res 159:285–293. CrossRefGoogle Scholar
  33. Paez-Garcia A, Motes CM, Scheible WR, Chen R, Blancaflor EB, Monteros MJ (2015) Root traits and phenotyping strategies for plant improvement. Plants 4(2):334–355. CrossRefPubMedPubMedCentralGoogle Scholar
  34. Pang J, Ryan M, Siddique KHM, Simpson R (2017) Unwrapping the Rhizosheath. Plant Soil 418(1–2):129–139. CrossRefGoogle Scholar
  35. Price SR (1911) The Roots of Some North African Desert-Grasses 10 (9–10): 328–40. CrossRefGoogle Scholar
  36. Sharp RE, Poroyko V, Hejlek LG, Spollen WG, Springer GK, Bohnert HJ, Nguyen HT (2004) Root growth maintenance during water deficits: physiology to functional genomics. J Exp Bot 55(407):2343–2351. CrossRefPubMedGoogle Scholar
  37. Smith RJ, Hopper SD (2011) Shane MW sand-binding roots in haemodoraceae: global survey and morphology in a phylogenetic context. Plant Soil 348:453–470. CrossRefGoogle Scholar
  38. Tardieu F, Davies WJ (1992) Stomatal response to abscisic acid is a function of current plant water status. Plant Physiol 98:540–545. CrossRefPubMedPubMedCentralGoogle Scholar
  39. Vadez V (2014) Root hydraulics: the forgotten side of roots in drought adaptation. Field Crop Research 165: 15–24. 0.1016/j.fcr.2014.03.017CrossRefGoogle Scholar
  40. Wasaya A, Zhang X, Fang Q, Yan Z (2018) Root Phenotyping for drought tolerance: a review. Agronomy 8(11):241. CrossRefGoogle Scholar
  41. Watt M, McCully ME, Canny MJ (1994) Formation and stabilization of Rhizosheaths of Zea Mays L. (effect of soil water content). J Plant Physiol 106:179–186. CrossRefGoogle Scholar
  42. Young IM (1995) Variation in moisture contents between bulk soil and the rhizosheath of wheat (Triticum-aestivum Lcv Wembley). New Phytol 130:135–139. CrossRefGoogle Scholar
  43. Zarebanadkouki M, Meunier F, Couvreur V, Cesar J, Javaux M, Carminati A (2016) Estimation of the hydraulic conductivities of lupine roots by inverse modelling of high-resolution measurements of root water uptake. Ann Bot 118(4):853–864. CrossRefPubMedCentralGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Soil and Water Research InstituteAgricultural Research Education and Extension Organization (AREEO)KarajIran
  2. 2.Department of Agronomy and Plant BreedingUniversity of TehranTehranIran
  3. 3.Chair of Soil PhysicsUniversity of BayreuthBayreuthGermany

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