Long-term rice-rice-rape rotation optimizes 1,2-benzenediol concentration in rhizosphere soil and improves nitrogen-use efficiency and rice growth
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We examined differences in soil metabolites from the rice root rhizosphere of long-term rice-rice-fallow (RRF) and rice-rice-rape (RRR) rotations, and examined the effects of 1,2-benzenediol on nitrogen-use efficiency (NUE) and rice growth.
The metabolite composition of rice rhizospheres was analyzed using the gas chromatography-mass spectrometry (GC-MS). A range of 0.2, 2.0 and 200 μmol L−1 concentrations of external 1,2-benzenediol were applied to examine their effects on rice growth, nitrate reductase (NR) and glutamine synthetase (GS) activities, and physiological nitrogen-use efficiency (PNUE).
The metabolite composition of rhizospheres differed significantly between RRR and RRF. Soil total N and 1,2-benzenediol concentrations during the early rice season were significantly lower under RRR than RRF. Rice growth and NUE significantly enhanced at 0.20 μmol 1,2-benzenediol L−1, but inhibited at 2.0 μmol L−1 or higher. Changes in root morphology and uptake associated with 1,2-benzenediol possibly had contributed to a higher NUE of the early season rice under RRR. The NR and GS activities in rice roots were significantly higher with 0.2 μmol L−1 1,2-benzenediol than without 1,2-benzenediol treatment.
Crop rotation significantly affected rice rhizosphere metabolites. An optimal soil 1,2-benzenediol concentration under long-term RRR rotation may be associated with an enhanced NUE and root N uptake and assimilation, resulting in an increased rice growth and yield.
KeywordsAllelochemical metabolite Brassica napus Glutamine synthetase Nitrate reductase Oryza sativa
This study was supported in part by the National Key R&D Program of China (2017YFD0200100; 2017YFD0200104); National Natural Science Foundation of China (31101596, 31372130); Hunan Provincial Recruitment Program of Foreign Experts; the National Oilseed Rape Production Technology System of China; and the 2011 Plan of the Chinese Ministry of Education; and the Double First Class Construction Project of Hunan Agricultural University (kxk201801005).
- Bao SD (2000) Analytical methods of soil agro-chemistry. China Agriculture Press, Beijing (in Chinese)Google Scholar
- Gu Y, Chang ZZ, Yu JG, Zong LG (2013) Allelopathic effects of exogenous phenolic acids composted by wheat straw on seed germination and seedling growth of rice. Jiangsu J of Agr Sci 29:240–246 (in Chinese with English abstract)Google Scholar
- Jiang SF, Wang CJZ, Shu CW, Zhou EX (2018) Cloning and expression analysis of rsphm gene in rhizoctonia solani ag-1, a of rice sheath blight pathogen. Chin J Rice Sci 32:111–118 (in Chinese with English abstract)Google Scholar
- Liu P, Zhao HJ, Wan SB, Jiang LH, Yu SF, Yang L, Wang YQ, Li J (2010) Autotoxic potential of peanut (Arachis hypogaea) root exudates. Chinese Journal of Oil Crop Sciences 32:431–435 (in Chinese with English abstract)Google Scholar
- Lu S, Zhang ZH (2018) Long-term rice-rice-rape rotation significantly improved soil structure and rice yield [J]. Chinese Journal of Soil Science 49(2):409–414 (in Chinese with English abstract)Google Scholar
- Rice EL, Putnam AR, Tang CS (1986) Allelopathic growth stimulation. In: The science of Allelopathy. John Wiley& Sons, New York, pp 23–42Google Scholar
- Shilling DG, Yoshiiawa FA (1987) Seeding bioassay for the study of allelopathy in allelochemicals role in agriculture and forestry, Waller GR, Ed.. ACS Symp. Ser no. 330. American chemical society. Chap, Washington, D.C, p 31Google Scholar
- Wang H, Du NM (2006) Research and prospect of rice cropping system. Crop Res 5:498–503 (in Chinese with English abstract)Google Scholar
- Watanabe K, Teramoto M, Futamata H, Harayama S (1998) Molecular detection, isolation, and physiological characterization of functionally dominant phenol-degrading bacteria in activated sludge. Appl Environ Microb 64:4396–4402Google Scholar
- Wu CX, Li ZH, Shen YX (2007) Quantification and allelopathy potential of phenolic acids in aqueous extracts of legumes. Acta Agrestia Sintca 15:401–406 (in Chinese with English abstract)Google Scholar
- Yuan DG, Wu JQ, Zhai HK, Wu QQ, Deng H, Xu GX, Luo Q, Wu DY, Wang CQ (2012) Activation effects of N, P, K fertilization and phenol on Si, AI, Fe in bleached paddy soil. Plant Nutrition and Fertilizer Science 18:771–776 (in Chinese with English abstract)Google Scholar
- Zhou BM, Cui MY, Song JJ, Liu JL, Huang W, Yu GL, Liu RQ (2011) Effect of 1,2-benzenediol on the utilization rate of nitrogen fertilizer and rice yield. Modern Agricultural Science and Technology 17:248–252 (in Chinese with English abstract)Google Scholar