A new function of the biocontrol bacterium Lysobacter enzymogenes LE16 in the mineralization of soil organic phosphorus
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To evaluate the functions of a new biocontrol bacterium, Lysobacter enzymogenes LE16, in the mineralization of soil organic phosphorus (P) and in the stimulation of plant P uptake and plant growth.
Liquid culture, soil incubation, and pot experiments were used to study phosphatase production, inorganic P release from lecithin and soil organic P compounds, and the P uptake and growth of lettuce seedlings induced by L. enzymogenes LE16.
This bacterium hydrolyzed lecithin by the release of acid, neutral and alkaline phosphatases in culture solutions with a pH ranging from 4.0 to 10.0 at 12–36 °C. The hydrolysis reactions were unaffected by NH4+-N, NO3−-N or urea at equal N concentrations but were promoted by low inorganic P. Bacterial inoculation significantly decreased the organic P but increased the water-soluble P and Olsen P in the soil. Compared to the use of chemical fertilizers alone, the P uptake, biomass, and economic yield of lettuce increased by 16.41%, 11.58%, and 11.30%, respectively, with an increase in soil Olsen P in the L. enzymogenes LE16 plus chemical fertilizer treatment.
Environmental adaptation and phosphatase production could be the mechanisms involved in soil P mineralization and plant pathogen suppression by L. enzymogenes LE16. This is the first demonstration of a new use of L. enzymogenes LE16 in agriculture beyond plant protection.
KeywordsLysobacter enzymogenes Phosphatase Soil organic phosphorus Growth promotion
This research work was financially supported by the Chongqing Scientific Innovation Project for Postgraduates (Project CYB17064), Chongqing Science and Technology Committee (cstc2018jscx-mszd0133 and cstc2017shms-xdny80084), and Zunyi Tobacco Company (ZY 2015-03 and ZY 2018-01).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Human participants and/or animals
There is no research involving Human Participants and/or Animals in this article.
Informed consent was obtained from all individual participants included in the study.
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