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Effect of NaCl on proline and glycinebetaine metabolism in Kosteletzkya pentacarpos exposed to Cd and Zn toxicities

  • Ming-Xi Zhou
  • Marie-Eve Renard
  • Muriel Quinet
  • Stanley LuttsEmail author
Regular Article
  • 27 Downloads

Abstract

Background and aims

Proline and glycinebetaine are osmolytes playing a role in resistance to salt and water stress but their involvement in plant adaptation to heavy metals remain unclear.

Methods

Young plants of the halophyte Kosteletzkya pentacarpos were grown in nutrient solution in the presence of Cd (20 or 40 μM) or Zn (200 or 400 μM), or a combination of both heavy metals and in the presence or absence of NaCl 50 mM for 48 h. Osmolytes concentrations, enzyme activities involved in their metabolism and expression of corresponding genes were determined in roots and leaves.

Results

Cadmium but not zinc increased proline and glycinebetaine in the leaves. Salinity reduced proline content in Cd-treated plants but increased it in plants exposed to Cd + Zn. Proline was produced through both glutamate and ornithine pathways while proline dehydrogenase was inhibited in response to heavy metals. Correlation between enzyme activities and corresponding gene expression was significant in the leaves but not in the roots. Gene coding for proline transport (KvProT) was upregulated in response to heavy metals.

Conclusion

Low NaCl dose (50 mM) afford protection to heavy metal stress in K. pentacarpos and its effect on osmolyte synthesis depends on considered metal and plant organ.

Keywords

Halophyte Heavy metals Phytoremediation Salinity Seashore mallow Wetland 

Notes

Acknowledgements

The authors wish to thank Dr. P. Qin (University of Nanjing) for providing the seeds. Mingxi ZHOU is grateful to the CSC (China scholarship council) for the award of a research fellowship. This article is devoted to the memory of Professor Gilles Guerrier (Université d’Orléans, France).

Supplementary material

11104_2019_4143_MOESM1_ESM.docx (76 kb)
ESM 1 (DOCX 76 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Ming-Xi Zhou
    • 1
  • Marie-Eve Renard
    • 1
  • Muriel Quinet
    • 1
  • Stanley Lutts
    • 1
    Email author
  1. 1.Groupe de Recherche en Physiologie végétaleEarth and Life Institute -Agronomy (ELI-A; Université catholique de Louvain)Louvain-la-NeuveBelgium

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