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Dark septate endophytes improve the growth of host and non-host plants under drought stress through altered root development

  • Xia Li
  • Chao He
  • Xueli HeEmail author
  • Fang Su
  • Lifeng Hou
  • Ying Ren
  • Yiting Hou
Regular Article
  • 70 Downloads

Abstract

Aims

This study aimed to investigate how dark septate endophytes (DSE) from arid habitats affect host growth and their application to crops and medicinal plants in drought-prone soils.

Methods

First, the osmotic-stress tolerance of Paraphoma sp., Embellisia chlamydospora, and Cladosporium oxysporum, isolated from Hedysarum scoparium, was tested using osmotically adjusted pure culture. Second, we examined the performance of host (H. scoparium) and non-host (Glycyrrhiza uralensis and Zea mays) plants inoculated with these fungi under mild (MD) and extreme drought (ED) conditions in a growth chamber.

Results

All the DSE showed high tolerance to osmotic stress in vitro and could colonise the roots of all the plants. For H. scoparium, DSE improved the root biomass and length depending on DSE species, with Paraphoma sp. and C. oxysporum exhibiting positive effects under all the drought treatments. For G. uralensis and Z. mays, DSE inoculation enhanced the root development of plants under MD condition and was dependent on the plant–fungus species. However, this positive effect was weakened under extreme drought stress.

Conclusions

DSE isolated from H. scoparium enhanced the root growth of the host plant under drought conditions and may also be used to promote the cultivation of agricultural and medicinal plants.

Keywords

Hedysarum scoparium Dark septate endophytes (DSE) Root development Drought Symbiosis 

Notes

Acknowledgements

This study was financially supported by the National Natural Science Foundation of China (project no. 31470533, 31770561, 31800345). We greatly appreciate the support of Experimental Center of Desert Forestry, CAF for their invaluable assistance on this experiment.

Supplementary material

11104_2019_4057_MOESM1_ESM.docx (1.2 mb)
ESM 1 (DOCX 1183 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Xia Li
    • 1
  • Chao He
    • 2
  • Xueli He
    • 1
    Email author
  • Fang Su
    • 1
  • Lifeng Hou
    • 1
  • Ying Ren
    • 1
  • Yiting Hou
    • 1
  1. 1.College of Life SciencesHebei UniversityBaodingChina
  2. 2.Institute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences & Peking Union Medical CollegeBeijingChina

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