Plant and Soil

, Volume 437, Issue 1–2, pp 195–213 | Cite as

High intrinsic seed Zn concentration improves abiotic stress tolerance in wheat

  • Muhammad Faran
  • Muhammad FarooqEmail author
  • Abdul Rehman
  • Ahmad Nawaz
  • Muhammad Kamran Saleem
  • Nauman Ali
  • Kadambot H. M. Siddique
Regular Article



Abiotic stresses are threatening wheat productivity across the globe, which is often associated with nutrient deficiencies. Zinc (Zn) is involved in many physiological processes of plants, and high intrinsic seed Zn concentrations may help to improve the resistance of wheat to abiotic stresses.


Three separate experiments evaluated the effect of intrinsic seed zinc on bread wheat resistance to abiotic stresses, viz. waterlogging, drought and salinity. One-week-old wheat seedlings raised from seeds containing either 49 mg (high), 42 mg (medium), or 35 mg (low) Zn kg−1 grain were exposed to waterlogging or drought stress for one week or until harvest. Salinity stress was applied at sowing for one week or until harvest.


Plants with high intrinsic seed Zn performed better than those with medium or low Zn concentrations under each stress, including lower malondialdehyde contents and total antioxidant activities and more proline. The grain yield in plants from high, medium and low seed Zn concentrations increased by 10.5–48%, 12.2–21.5% and 7.7–21% under waterlogging, drought and salinity stress, respectively.


Plants with high intrinsic seed Zn concentrations produced higher wheat grain yields than those with lower levels under abiotic stress by reducing oxidative damage and improving the growth and uptake of nutrients.


Salinity Waterlogging Drought Nitrogen Potassium Seed Zn Stay-green Root growth 


Supplementary material

11104_2019_3977_MOESM1_ESM.docx (20 kb)
ESM 1 (DOCX 20 kb)


  1. Ahmad P (2012) Salt-induced changes in photosynthetic activity and oxidative defense system of three cultivars of mustard (Brassica juncea L.). Afr J Biotechnol 11:2694–2703Google Scholar
  2. Ahmad P, Jaleel CA, Salem MA, Nabi G, Sharma S (2010) Roles of enzymatic and nonenzymatic antioxidants in plants during abiotic stress. Crit Rev Biotechnol 30:161–175CrossRefGoogle Scholar
  3. Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207CrossRefGoogle Scholar
  4. Bremner JM, Mulvaney CS (1982) Total nitrogen. In: Page AL, Miller RH, Keeny DR (eds) Methods of soil analysis. Am Soc Agron Soil Sci Soc Am Madison, pp 1119–1123Google Scholar
  5. Cakmak I (2008) Enrichment of cereal grains with zinc: agronomic or genetic biofortification? Plant Soil 302:1–17CrossRefGoogle Scholar
  6. Cakmak I, Marschner H (1993) Effect of zinc nutritional status on activities of superoxide radical and hydrogen peroxide scavenging enzymes in bean leaves. In: Plant Nutrition — from Genetic Engineering to Field Practice. Springer Netherlands, Dordrecht, pp 133–136CrossRefGoogle Scholar
  7. Cakmak I, Marschner H (1998) Increase in membrane permeability and exudation in roots of Zn deficient plants. J Plant Physiol 132:356–361CrossRefGoogle Scholar
  8. Chapman SC, Barreto HJ (1995) Using a chlorophyll meter to estimate specific leaf nitrogen of tropical maize during vegetative growth. Agron J 89:557–562CrossRefGoogle Scholar
  9. Chaves MM, Oliveira MM (2004) Mechanisms underlying plant resilience to water deficits: prospects for water-saving agriculture. J Exp Bot 55:2365–2384CrossRefPubMedGoogle Scholar
  10. Cheryl M (2012) What causes floods? Online available at Accessed 1 July 2018
  11. Cox AE, Joern BC, Brouder SM, Gao D (1999) Plant-available potassium assessment with a modified sodium tetraphenyl boron method. Soil Sci Soc Am J 63:902–911CrossRefGoogle Scholar
  12. Das L (2000) Problems facing plant breeding CBS Publishers and Distributors, New DelhiGoogle Scholar
  13. Estefan G, Sommer R, Ryan J (2013) Methods of soil, plant and water analysis: a manual for the West Asia and North Africa Region. 3rd edn. ICARDA, West Asia, pp 61–133Google Scholar
  14. Guo JX, Feng XM, Hu XY et al (2015) Effects of soil zinc availability, nitrogen fertilizer rate and zinc fertilizer application method on zinc biofortification of rice. J Agric Sci 154:584–597Google Scholar
  15. Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts: I Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198Google Scholar
  16. IPCC (2007) Intergovernmental Panel on Climate Change Intergovernmental Panel on Climate Change fourth assessment report: Climate change 2007. Synthesis Report; World Meteorological Organization; Geneva; Switzerland. ipcc.chGoogle Scholar
  17. Julkunen-Tiitto R (1985) Phenolic constituents in the leaves of northern willows: methods for the analysis of certain phenolics. J Agric Food Chem 33:213–217CrossRefGoogle Scholar
  18. Kreuzwieser J, Hauberg J, Howell KA et al (2009) Differential response of gray poplar leaves and roots underpins stress adaptation during hypoxia. Plant Physiol 149:461–473CrossRefPubMedPubMedCentralGoogle Scholar
  19. Lee DJ, Kim KH, Kang JH, Lee YS, Kim HW (2006) Antioxidant and anticancer activities of methanolic extracts in grains of the Korean rice landraces. J Korean Soc Food Sci Nutr 18:264–269Google Scholar
  20. Lindsay WL, Norvell WA (1978) Development of a DTPA soil test for zinc, iron, manganese, and copper. Soil Sci Soc Amer J 42:421–428CrossRefGoogle Scholar
  21. Lopez CG, Banowetz GM, Peterson CJ, Kronstad WE (2003) Dehydrin expression and drought tolerance in seven wheat cultivars. Crop Sci 43:577–582CrossRefGoogle Scholar
  22. Mahajan S, Tuteja N (2005) Cold, salinity and drought stresses: an overview. Arch Biochem Biophys 444:139–158CrossRefPubMedGoogle Scholar
  23. Olsen SR, Cole CV, Watanabe FS, Dean LA (1954) In: Banderis AD, Barter DH, Anderson K (eds) Estimation of Available Phosphorus in Soils by Extraction with Sodium Bicarbonate. U.S. Department of Agriculture (Circular No. 939. Agricultural and Advisor)Google Scholar
  24. Ozturk A, Caglar O, Bulut S (2006) Growth and yield response of facultative wheat to winter sowing, freezing sowing and spring sowing at different seeding rates. J Agron Crop Sci 192:10–16CrossRefGoogle Scholar
  25. Pongrac P, McNicol JW, Lilly A, Thompson JA, Wright G, Hillier S, White PJ (2018) Mineral element composition of cabbage as affected by soil type and phosphorus and zinc fertilisation. Plant Soil.
  26. Rehman A, Farooq M, Ozturk L, Asif M, Siddique KH (2018a) Zinc nutrition in wheat-based cropping systems. Plant Soil 422:283–315CrossRefGoogle Scholar
  27. Rehman A, Farooq M, Naveed M, Ozturk L, Nawaz A (2018b) Pseudomonas-aided zinc application improves the productivity and biofortification of bread wheat. Crop Pasture Sci 69:659–672CrossRefGoogle Scholar
  28. Rehman A, Farooq M, Naveed M, Nawaz A, Shahzad B (2018c) Seed priming of Zn with endophytic bacteria improves the productivity and grain biofortification of bread wheat. Eur J Agron 94:98–107CrossRefGoogle Scholar
  29. Rengel Z, Graham RD (1995) Wheat genotypes differ in zinc efficiency when grown in the chelate-buffered nutrient solution. Growth Plant Soil 176:307–316Google Scholar
  30. Rennenberg H, Loreto F, Polle A, Brilli F, Fares S, Beniwal RS, Gessler A (2006) Physiological responses of forest trees to heat and drought. Plant Biol 8:556–571CrossRefPubMedGoogle Scholar
  31. Richards L (1954) Diagnosis and improvement of saline and alkali soils. USDA Agric. Handbook 60. Washington, DCGoogle Scholar
  32. Rincon M, Hanson JB (1986) Control of calcium ion fluxes in injured or shocked corn root cells: importance of proton pumping and cell membrane potential. Physiol Plant 67:576–583CrossRefGoogle Scholar
  33. Shahryari R, Mollasadeghi V (2011a) Correlation study of some traits affecting yield and yield components of wheat genotypes in terms of normal irrigation and end drought stress. Adv Environ Biol:523–528Google Scholar
  34. Shahryari R, Mollasadeghi V (2011b) Introduction of two principal components for screening of wheat genotypes under end seasonal drought. Adv Environ Biol:519–523Google Scholar
  35. Steel RGD, Torrie JH, Dickey DA (1996) Principles and procedures of statistics: a biometrical approach, 3rd edn. McGraw Hill Book Co. Inc., New YorkGoogle Scholar
  36. Tavallali V, Rahemi M, Eshgi S et al (2010) Zinc alleviates salt stress and increases antioxidant enzyme activity in the leaves of pistachio (Pistacia vera L. Badami) seedlings. Turk J Agri For 34:349–359Google Scholar
  37. White PJ, Veneklaas EJ (2012) Nature and nurture: the importance of seed phosphorus content. Plant Soil 357:1–8CrossRefGoogle Scholar
  38. Xue Q, Rudd JC, Liu S et al (2014) Yield determination and water-use efficiency of wheat under water-limited conditions in the U.S. Southern High Plains. Crop Sci 54:34–47CrossRefGoogle Scholar
  39. Yilmaz A, Ekiz H, Gültekin I, Torun B, Barut H, Karanlik S, Cakmak I (1998) Effect of seed zinc content on grain yield and zinc concentration of wheat grown in zinc-deficient calcareous soils. J Plant Nutr 21:2257–2264CrossRefGoogle Scholar
  40. Zhang YQ, Deng Y, Chen RY, Cui ZL, Chen XP, Yost R, Zhang FS, Zou CQ (2012) The reduction in zinc concentration of wheat grain upon increased phosphorus-fertilization and its mitigation by foliar Zn application. Plant Soil 361:143–152CrossRefGoogle Scholar

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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Department of AgronomyUniversity of AgricultureFaisalabadPakistan
  2. 2.Department of Boreal Ecosystems and Agricultural Sciences, School of Science and the EnvironmentMemorial University of NewfoundlandSt. John’sCanada
  3. 3.Department of Crop Sciences, College of Agricultural and Marine SciencesSultan Qaboos UniversityMuscatOman
  4. 4.The UWA Institute of AgricultureThe University of Western AustraliaPerthAustralia
  5. 5.Department of Crop Science and BiotechnologyDankook UniversityChungnamRepublic of Korea
  6. 6.College of AgricultureBahauddin Zakariya UniversityLayyahPakistan
  7. 7.Agronomic Research Institute, Ayub Agricultural Research InstituteFaisalabadPakistan

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