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A unique aluminum resistance mechanism conferred by aluminum and salicylic-acid-activated root efflux of benzoxazinoids in maize

  • Zunkang Zhao
  • Xiaofeng Gao
  • Ye Ke
  • Minmin Chang
  • Lu Xie
  • Xiaofeng Li
  • Minghua Gu
  • Jiping LiuEmail author
  • Xinlian TangEmail author
Regular Article

Abstract

Background and aims

Although aluminum (Al) exclusion via root exudation of organic matters is a common resistance mechanism adopted by many plant species, whether root exudation of benzoxazinoids, such as hydroxamic acids (HAs), confers Al resistance remains unclear.

Methods

We performed physiological characterization for an Al-resistant maize cultivar TY and a sensitive maize cultivar ZD.

Results

First, Al exposure induced HA exudation from the root tip of TY, but not from ZD. Second, HAs formed non-toxic Al chelation complexes in vitro and exogenous HAs alleviated root damage and improved root growth under Al stresses. Third, both Al and exogenous salicylic acid (SA) treatments induced accumulation of endogenous SAs in the root apices of TY, which in turn enhanced root HA exudation and Al resistance in TY. Furthermore, an SA biosynthesis inhibitor significantly decreased Al resistance in TY and abolished the beneficial effects of exogenous SA on Al resistance, suggesting a key role of the endogenous SAs in induction of Al resistance. Finally, it was the root-tip HA exudation but not the root-tip HA contents that determined Al resistance in maize.

Conclusion

We have revealed a unique Al exclusion mechanism underlying Al resistance via Al and SA-mediated root HA efflux in maize.

Keywords

Aluminum tolerance Benxozaxinoids Hydroxamic acids Maize Root exudate Salicylic acid 

Notes

Acknowledgements

This study is supported by the National Natural Science Foundation of China (grant NO. 31201680) and Guangxi Natural Science Foundation (grant NO. 2012GXNSFAA053047 and NO. 2016GXNSFAA380230).

Author’s contribution

This study was conceived and supervised by XL, MG, ZZ, JL and XT; XG, YK, MC and LX performed the experiments. The manuscript was written by ZZ, XT and JL and was reviewed by all authors.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11104_2019_3971_MOESM1_ESM.docx (559 kb)
ESM 1 (DOCX 559 kb)

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Authors and Affiliations

  1. 1.State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Cultivation Base of Guangxi Key Laboratory for Agro-Environment and Agro-Product Safety, College of AgricultureGuangxi UniversityNanningChina
  2. 2.National Demonstration Center for Experimental Plant Science EducationGuangxi UniversityNanningChina
  3. 3.United States Department of Agriculture, Agricultural Research ServiceRobert W. Holley CenterIthacaUSA

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