Plant and Soil

, Volume 437, Issue 1–2, pp 11–20 | Cite as

Antimony tolerance and accumulation in a metallicolous and a non-metallicolous population of Salvia spinosa L.

  • Shakiba Rajabpoor
  • Seyed Majid GhaderianEmail author
  • Henk Schat
Regular Article



Antimony (Sb) is locally found at potentially toxic concentrations in mineralized soils, usually together with arsenic (As). However, local adaptation of plant populations to Sb toxicity has never been shown thus far. Here we compared Sb tolerance and accumulation between a non-metallicolous (NM) population of Salvia spinosa, and a con-specific metallicolous (M) population from a strongly Sb/As-enriched soil in Dashkasan, Iran.


Plants were exposed in hydroponics to a series of Sb[III] and Sb[V] concentrations. After 3 weeks the dry weights and Sb concentrations of roots and shoots were determined.


Estimated from the effects on shoot dry weight, the M population was more tolerant than the NM one, particularly to Sb[V], but to a lower degree also to Sb[III]. In both populations Sb[III] was taken up and translocated at higher rates than Sb[V]. The Sb concentrations in roots and shoots were slightly, but significantly higher in the M than in the NM population.


Since Sb[V] and As[V] seem to follow very different detoxification pathways, it can be argued that the superior tolerance to Sb[V] in M represents a local adaptation to Sb[V] toxicity itself, rather than being a mere by-product of hypertolerance to its chemical analogue, As[V]. Since Sb[III] and As[III] or As[V] share common detoxification pathways, the apparent Sb[III] hypertolerance in the M population might represent a by-product of As hypertolerance.


Antimony Arsenic Accumulation Tolerance Salvia spinosa Dashkasan 



We would like to thank the Graduate School of University of Isfahan for providing research facilities for this study.


  1. Anawar HM, Akai J, Mihaljevič M, Sikder AM, Ahmed G, Tareq S, Rahman MM (2011) Arsenic contamination in groundwater of bangladesh: perspectives on geochemical, microbial and anthropogenic. Water 3:1050–1076CrossRefGoogle Scholar
  2. Antonovics J, Bradshaw AD, Turner AG (1971) Heavy metal tolerance in plants. Adv Ecol Res 7:1–8CrossRefGoogle Scholar
  3. Baker AJM, Brooks RR, Pease AJ, Malaisse F (1983) Studies on copper and cobalt tolerancde in 3 closely related taxa within the genus Silene L. (Caryophyllaceae) from Zaire. Plant Soil 73:377–385CrossRefGoogle Scholar
  4. Bleeker PM, Hakvoort HWJ, Bliek M, Souer E, Schat H (2006) Enhanced arsenate reduction by a CDC25-like tyrosine phosphatase explains increased phytochelatin accumulation in arsenate-tolerant Holcus lanatus. Plant J 45:917–929CrossRefPubMedGoogle Scholar
  5. Casado M, Anawar HM, Garcia-Sanchez A, Santa Regina I (2007) Sb and arsenic uptake by plants in an abandoned mining area. Commun Soil Sci Plant Anal 38:1255–1275CrossRefGoogle Scholar
  6. Chao DY, Chen Y, Chen J, Shi S, Chen Z, Wang C, Danku J, Zhao FJ, Salt DE (2014) Genome-wide association mapping identifies a new arsenate reductase enzyme critical for limiting arsenic accumulation in plants. PLoS Biol 12:1–17CrossRefGoogle Scholar
  7. Clemens S (2001) Molecular mechanisms of plant metal tolerance and homeostasis. Planta 212:475–486CrossRefPubMedGoogle Scholar
  8. Corrales I, Barceló J, Bech J, Poschenrieder C (2014) Antimony accumulation and toxicity mechanisms in Trifolium species. J Geochem Explor 147:167–172CrossRefGoogle Scholar
  9. Crommentuijn T, Polder MD, van de Plassche EJ (1997) Maximum permissible concentrations and negligible concentrations of metals, taking background concentrations into account. National Institute of Public Health and the Environment, Bilthoven, the Netherlands. RIVM Report No. 601 501 001Google Scholar
  10. Ernst WHO, Verkleij JAC, Schat H (1992) Metal tolerance in plants. Acta Bot Neerl 41:229–248CrossRefGoogle Scholar
  11. Filella M, Williams PA, Belzile N (2009) Antimony in the environment: knowns and unknowns. Montserrat Environ Chem 6:95–105CrossRefGoogle Scholar
  12. Gregory RPG, Bradshaw AD (1965) Heavy metal tolerance in populations of agrostis tenuis Sibth. and other grasses. New Phytol 64:131–143CrossRefGoogle Scholar
  13. Hogan GD, Rauser WE (1979) Tolerance and toxicity of cobalt, copper, nickel and zinc in clones of Agrostis gigantea. New Phytol 83(3):665–670CrossRefGoogle Scholar
  14. Jamali Hajiani N, Ghaderian SM, Karimi N, Schat H (2015) A comparative study of Sb accumulation in plants growing in two mining areas in Iran, Moghanlo, and Patyar. Environ Sci Pollut Res 22:16542–16553CrossRefGoogle Scholar
  15. Jamali Hajiani N, Ghaderian SM, Karimi N, Schat H (2017) A comparison of antimony accumulation and tolerance among Achillea wilhelmsii, Silene vulgaris and Thlaspi arvense. Plant Soil 412:267–281CrossRefGoogle Scholar
  16. Kabata-Pendias A, Mukherjee AB (2007) Trace elements from soil to human. Springer–Verlag, BerlinCrossRefGoogle Scholar
  17. Kabata-Pendias A, Pendias H (2010) Trace elements in soils and plants. CRC Press, Boca RatonCrossRefGoogle Scholar
  18. Karimi N, Ghaderian SM, Schat H (2013) Arsenic in soil and vegetation of a contaminated area. Int J Environ Sci Technol 10:743–752CrossRefGoogle Scholar
  19. Le Faucheur S, Schildknecht F, Behra R, Sigg L (2006) Thiols in Scenedesmus vacuolatus upon exposure to metals and metalloids. Aquat Toxicol 80:355–361CrossRefPubMedGoogle Scholar
  20. Li XD, Thornton I (1993) Arsenic, antimony and bismuth in soil and pasture herbage in some old metalliferous mining areas in England. Environ Geochem Health 15:135–144CrossRefPubMedGoogle Scholar
  21. Li Y, Sperry JS, Shao M (2009) Hydraulic conductance and vulnerability to cavitation in corn (Zea mays L.) hybrids of differing drought resistance environmental and experimental. Environ Exp Bot 66:341–346CrossRefGoogle Scholar
  22. Macnair MR (1983) The genetic control of copper tolerance in the yellow monkey flower, Mimulus guttatus. Heredity 50:283–293CrossRefGoogle Scholar
  23. Macnair MR (1993) The genetics of metal tolerance in vascular plants. New Phytol 124:541–559CrossRefGoogle Scholar
  24. Macnair MR, Cumbes QJ, Meharg AA (1992) The genetics of arsenate tolerance in Yorkshire fog, Holcus lanatus L. Heredity 69:325–335CrossRefGoogle Scholar
  25. Meharg AA, Jardine L (2003) Arsenic transport into paddy rice (Oryza sativa) roots. New Phytol 157:39–44CrossRefGoogle Scholar
  26. Meharg AA, Macnair MR (1992) Suppression of the high-affinity phosphate uptake system – a mechanism of arsenate tolerance in Holcus lanatus L. J Exp Bot 43:519–524CrossRefGoogle Scholar
  27. Moritz R, Ghazban F, Singer BS (2006) Eocene gold ore formation at Muteh, Sanandaj-Sirjan extension and exhumation of metamorphic basement rocks within the Zagros orogen. Econ Geol 101:1497–1524CrossRefGoogle Scholar
  28. Perez-Sirvent C, Martinez-Sanchez MJ, Martinez-Lopez S, Bech J, Bolan N (2012) Distribution and bioaccumulation of arsenic and antimony in Dittrichia viscosa growing in mining-affected semiarid soils in Southeast Spain. J Geochem Explor 123:128–135CrossRefGoogle Scholar
  29. Raab A, Feldmann J, Meharg AA (2004) The nature of arsenic-phytochelatin complexes in Holcus lanatus and Pteris cretica. Am Soc Plant Biol 134:1113–1122Google Scholar
  30. Ren J, Pei-Chen Lin C, Pathak MC, Temple BR, Nile AH, Mousley CJ, Duncan MC, Eckert DM, Leiker TJ, Ivanova PT, Myers DS, Murphy RC, Brown HA, Verdaasdonk J, Bloom KS, Ortlund EA, Neiman AM, Bankaitis VA (2014) A phosphatidylinositol transfer protein integrates phosphoinositide signaling with lipid droplet metabolism to regulate a developmental program of nutrient stress-induced membrane biogenesis. Mol Biol Cell 25:712–727CrossRefPubMedPubMedCentralGoogle Scholar
  31. Schat H, Ten Bookum WM (1992) Genetic control of copper tolerance in Silene vulgaris. Heredity 68:219–229CrossRefGoogle Scholar
  32. Schat H, Vooijs R (1997) Multiple tolerance and co-tolerance to heavy metals in Silene vulgaris: a co-segregation analysis. New Phytol 136(3):489–496CrossRefGoogle Scholar
  33. Schat H, Kuiper E, Ten Bookum WM, Vooijs R (1993) General model for the genetic control of copper tolerance in Silene vulgaris: evidence from crosses between plants from different tolerant populations. Heredity 70:142–147CrossRefGoogle Scholar
  34. Schat H, Vooijs R, Kuiper E (1996) Identical major gene loci for heavy metal tolerances that have independently evolved in different local populations and subspecies of Silene vulgaris. Evolution 50(5):1888–1895CrossRefPubMedGoogle Scholar
  35. Sokal RR, Rohlf FJ (1981) Biometry, 2nd edn. WH Freeman and company, San FranciscoGoogle Scholar
  36. Song W-Y, Park J, Mendoza-Cozatl DG, Suter-Grotemeyer M, Shim D, Hortensteiner S, Geisler M, Weder B, Rea PA, Rentsch D, Schroedewr JI, Lee Y, Martinoia E (2010) Arsenic tolerance in Arabidopsis is mediated by two ABCC-type phytochelatin transporters. Proc Nat Acad Sc USA 107:21187–21192CrossRefGoogle Scholar
  37. Tilstone G, Macnair MR (1997) Nickel tolerance and copper-nickel co-tolerance in Mimulus guttatus from copper mine and serpentine habitats. Plant Soil 191(2):173–180CrossRefGoogle Scholar
  38. Tisarum R, Lessl JT, Dong X, de Oliveira LM, Rathinasabapathi B, Ma LQ (2014) Antimony uptake, efflux and speciation in arsenic hyperaccumulator Pteris vittata. Environ Pollut 186:110–114CrossRefPubMedGoogle Scholar
  39. Tschan M, Robinson BH, Schulin R (2009) Antimony in the soil–plant system – a review. Environ Chem 6:106–115CrossRefGoogle Scholar
  40. Van der Ent A, Baker AJM, van Balgooy MMJ, Tjoa A (2013) Ultramafic nickel laterites in Indonesia (Sulawesi, Halmahera): mining, nickel hyperaccumulators and opportunities for phytomining. J Geochem Explor 128:72–79CrossRefGoogle Scholar
  41. Vithanage M, Dabrowska BB, Mukherjee AB, Sandhi A, Bhattacharya P (2011) Arsenic uptake by plants and possible phytoremediation applications: a brief overview. Environ Chem Lett 10:217–224CrossRefGoogle Scholar
  42. Wysocki R, Clemens S, Augustyniak D, Golik P, Maciaszczyk E, Tamás MJ, Dziadkowiec D (2003) Metalloid tolerance based on phytochelatins is not functionally equivalent to the arsenite transporter Acr3p. Biochem Biophys Res Commun 304(2):293–300CrossRefPubMedGoogle Scholar

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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Department of Biology, Faculty of SciencesUniversity of IsfahanIsfahanIran
  2. 2.Department of Ecological Science, Faculty of SciencesVrije UniversiteitAmsterdamThe Netherlands

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