Marginal impact of cropping BADH transgenic maize BZ-136 on chemical property, enzyme activity, and bacterial community diversity of rhizosphere soil

  • Xin Bai
  • Xing Zeng
  • Siqi Huang
  • Jinsong Liang
  • Liying Dong
  • Yingnan Wei
  • Yue Li
  • Juanjuan QuEmail author
  • Zhenhua WangEmail author
Regular Article



Transgenic betaine aldehyde dehydrogenase (BADH) maize that overaccumulates glycine betaine (GB) is developed to enhance tolerance to salt stress, while the ecological risk of cropping BADH transgenic maize BZ-136 on soil properties and rhizosphere microorganisms is ambiguous.


A pot experiment was conducted and soils were sampled at seedling, elongation, flowering, and mature stage. Soil chemical properties and enzyme activities were determined with conventional method and bacterial community diversity of BZ-136 rhizosphere was analyzed by high-throughput sequencing technique as compared with those of parental maize Zheng58 and conventional maize U8112.


Cropping BZ-136 has a transient effect on EC, organic C or total N at some growth stage in neutral and saline-alkaline soil, a significant effect on urease activities from elongation to mature stage in saline-alkaline soil, and a slight influence on bacterial diversity at different stages in neutral soil and a significant impact at seedling stage in saline-alkaline soil.


Cropping BADH transgenic maize has transient or minor effects on soil chemistry, enzyme activity, and bacterial community diversity, while parallel factors, such as plant growth stage and soil type might also influence the rhizosphere microorganisms.


BADH transgenic maize Soil chemical property Soil enzyme activity Bacterial community Risk assessment 



Xin Bai and Xing Zeng contributed equally to this work. This work is supported by Major Subject of Environmental Safety Assessment Technology for Genetically Modified Maize, Wheat, and Soybean, China (No.2016ZX08011-003).

Supplementary material

11104_2019_3941_MOESM1_ESM.docx (608 kb)
ESM 1 (DOCX 608 kb)


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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.College of Resources and EnvironmentNortheast Agricultural UniversityHarbinChina
  2. 2.College of AgricultureNortheast Agricultural UniversityHarbinChina

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