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Plant and Soil

, Volume 432, Issue 1–2, pp 373–387 | Cite as

Involvement of auxin in the regulation of ammonium tolerance in rice (Oryza sativa L.)

  • Dong-Wei Di
  • Li Sun
  • Xiaonan Zhang
  • Guangjie Li
  • Herbert J. Kronzucker
  • Weiming Shi
Regular Article
  • 168 Downloads

Abstract

Background and aims

Ammonium (NH4+) is an important nitrogen source and is widely used as a fertilizer in agricultural systems. However, excess NH4+ inhibits root growth, and, subsequently, vegetative shoot growth and yield. This study examines whether auxin is involved in differential NH4+ tolerance in rice (Oryza sativa L.), and how auxin is regulated under high-NH4+ conditions in rice.

Methods

An NH4+-sensitive (Kasalath, Kas) and an NH4+-insensitive (Koshihikari, Kos) rive cultivar were cultured hydroponically with or without exogenous indole-3-acetic acid (IAA) and auxin biosynthesis inhibitors. Root growth, root area, tissue IAA content, and transcription of genes involved in auxin biosynthesis, conjugation and degradation were determined.

Results

pDR5::GUS staining and auxin measurement show that high NH4+ can decrease free IAA content in roots. In addition, quantitative RT-PCR, pharmacology, and genetics analysis suggest that Kos possesses a higher capacity for auxin biosynthesis and a weaker capacity for auxin metabolism compared to Kas under high-NH4+ stress.

Conclusion

We conclude that the NH4+-tolerant cultivar possesses a higher capacity to maintain auxin homeostasis under high-NH4+ stress, and that this advantage is incurred by promotion of auxin biosynthesis and a suppression of auxin metabolism.

Keywords

Ammonium toxicity Auxin level Nitrogen fertilizer Rice Root development 

Abbreviations

DAO

Dioxygenase for Auxin Oxidation

GH3

Group II GRETCHEN HAGEN3 acyl amido synthetases

IAA

indole-3-acetic acid

IAGLU

INDOLE-3-ACETATE BETA-D-GLUCOSYLTRANSFERASE

IPyA

indole-3-pyruvate acid

Kyn

L-kynurenine

NH4+

Ammonium

OxIAA

2-oxoindole-3-acetic acid

TAR

TRYPTOPHAN AMINOTRANSFERASE RELATED

Tryptophan

Trp

YUC

YUCCA

Yucasin

5-(4-chlorophenyl)-4H-1, 2, 4-triazole-3-thiol

Notes

Acknowledgements

We thank Prof. Shiping Wang (Huazhong Agricultural University) and Prof. Jianmin Wan (Nanjing Agricultural University) for kindly providing the OsGH3.2ox and Osdao mutant, respectively. This work was supported by grants from the National Natural Science Foundation of China [31430095, 31601823 and 31471948.], China Postdoctoral Science Foundation [2015 M58048 and 2017 T100411] and the University of Melbourne.

Supplementary material

11104_2018_3813_MOESM1_ESM.docx (1.2 mb)
ESM 1 (DOCX 1246 kb)
11104_2018_3813_MOESM2_ESM.xls (34 kb)
Table S1 Genes and rice cultivars used in this study. (XLS 33 kb)
11104_2018_3813_MOESM3_ESM.xls (37 kb)
Table S2 Primers used in qRT-PCR. (XLS 37 kb)
11104_2018_3813_MOESM4_ESM.xls (32 kb)
Table S3 Quantitative data of of shoots and roots in 25 cultivars under NA and HA. (XLS 32 kb)

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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil ScienceChinese Academy of SciencesNanjingChina
  2. 2.University of Chinese Academy of SciencesBeijingChina
  3. 3.School of Agriculture and Food, Faculty of Veterinary and Agricultural SciencesThe University of MelbourneParkvilleAustralia

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