Nodulation promotes cluster-root formation in Lupinus albus under low phosphorus conditions
- 69 Downloads
Background and aims
Phosphorus deficiency often limits crop productivity, while phosphate rock, which is used to produce phosphorus fertilisers, is a non-renewable resource. Formation of cluster roots is an adaptation to nutrient-poor soils in Lupinus species, including L. albus. Lupinus species also produce nodules, which require a large investment of phosphorus. Our aim is to test whether nodulation promotes cluster-root formation in L. albus.
Seedlings of L. albus, either inoculated with rhizobia or non-inoculated, were grown in nutrient solution with a low phosphorus supply. Non-inoculated plants were provided with the same amount of nitrogen in the form of nitrate as the nodulated ones acquired, from both air and nutrient solution, based on preliminary experiments. We measured biomass, phosphorus and nitrogen concentrations as well as photosynthesis just prior to each harvest.
Nodulated plants and non-nodulated control plants produced the same amount of biomass. Nodulated plants had, on average, 86% more cluster roots than non-nodulated ones at the four harvests. As hypothesised, nodulation significantly promoted cluster-root formation; it also enhanced rates of photosynthesis.
Nodulation promoted cluster-root formation and photosynthesis, presumably because nodules are significant sinks for phosphorus and photosynthates. Our results do not provide evidence for a trade-off between investment of resources in nodules and cluster roots.
KeywordsNitrogen fixation nodules proteoid roots rhizobium white lupin trade-off
We thank the editor and two reviewers for their constructive comments and advises on our manuscript. We are grateful for the help provided by Albina Ilyasova, Greg Cawthray and Haijie Zhang in Australia and by Kun Zhang, Li Shen as well as professor Weihua Guo in China. We also thank Jiayin Pang for her internal review of our manuscript. We thank the University of Western Australia and the Institute of Agriculture for support towards the research and the China Scholarship Council for a scholarship for Xiao Wang.
- Anonymous (1977) Technicon Industrial Method No. 334-74W/B+. Technicon Industrial Systems Tarrytown, NYGoogle Scholar
- Lambers H, Ahmedi I, Berkowitz O, Dunne C, Finnegan P M, Hardy GE, Jost R, Laliberte E, Pearse SJ, Teste FP (2013a) Phosphorus nutrition of phosphorus-sensitive Australian native plants: threats to plant communities in a global biodiversity hotspot. Conserv Physiol 1: cot010Google Scholar
- Lambers H, Atkin OK, Millenaar FF (2002) Respiratory patterns in roots in relation to their functioning. In: Waisel Y, Eshel A, Kafkaki U (eds) Plant roots The hidden half. 3rd edn. edn. Marcel Dekker, Inc., New YorkGoogle Scholar
- Raven JA (2013b) RNA function and phosphorus use by photosynthetic organisms. Front Plant Sci 4Google Scholar
- Rayment GE, Lyons DJ, Rayment GE, Lyons DJ (2010) Soil chemical methods: Australasia. CSIRO PublishingGoogle Scholar