Nodulation promotes cluster-root formation in Lupinus albus under low phosphorus conditions

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Abstract

Background and aims

Phosphorus deficiency often limits crop productivity, while phosphate rock, which is used to produce phosphorus fertilisers, is a non-renewable resource. Formation of cluster roots is an adaptation to nutrient-poor soils in Lupinus species, including L. albus. Lupinus species also produce nodules, which require a large investment of phosphorus. Our aim is to test whether nodulation promotes cluster-root formation in L. albus.

Methods

Seedlings of L. albus, either inoculated with rhizobia or non-inoculated, were grown in nutrient solution with a low phosphorus supply. Non-inoculated plants were provided with the same amount of nitrogen in the form of nitrate as the nodulated ones acquired, from both air and nutrient solution, based on preliminary experiments. We measured biomass, phosphorus and nitrogen concentrations as well as photosynthesis just prior to each harvest.

Results

Nodulated plants and non-nodulated control plants produced the same amount of biomass. Nodulated plants had, on average, 86% more cluster roots than non-nodulated ones at the four harvests. As hypothesised, nodulation significantly promoted cluster-root formation; it also enhanced rates of photosynthesis.

Conclusions

Nodulation promoted cluster-root formation and photosynthesis, presumably because nodules are significant sinks for phosphorus and photosynthates. Our results do not provide evidence for a trade-off between investment of resources in nodules and cluster roots.

Keywords

Nitrogen fixation nodules proteoid roots rhizobium white lupin trade-off 

Notes

Acknowledgements

We thank the editor and two reviewers for their constructive comments and advises on our manuscript. We are grateful for the help provided by Albina Ilyasova, Greg Cawthray and Haijie Zhang in Australia and by Kun Zhang, Li Shen as well as professor Weihua Guo in China. We also thank Jiayin Pang for her internal review of our manuscript. We thank the University of Western Australia and the Institute of Agriculture for support towards the research and the China Scholarship Council for a scholarship for Xiao Wang.

Supplementary material

11104_2018_3638_MOESM1_ESM.docx (17 kb)
ESM 1 (DOCX 16 kb)

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of Biological Sciences and Institute of AgricultureThe University of Western AustraliaPerthAustralia
  2. 2.Institute of Ecology and Biodiversity, School of Life SciencesShandong UniversityJinanPeople’s Republic of China

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