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Plant Molecular Biology

, Volume 101, Issue 6, pp 537–550 | Cite as

Evolution of MIR159/319 genes in Brassica campestris and their function in pollen development

  • Ziwei Hu
  • Xiuping Shen
  • Xun Xiang
  • Jiashu CaoEmail author
Article

Key Message

MIR159/319 have conserved evolution and diversified function after WGT in Brassica campestris, both of them can lead pollen vitality and germination abnormality, Bra-MIR319c also can function in flower development.

Abstract

MiR159 and miR319 are extensively studied highly conserved microRNAs which play roles in vegetative development, reproduction, and hormone regulation. In this study, the effects of whole-genome triplication (WGT) on the evolution of the MIR159/319 family and the functional diversification of the genes were comprehensively investigated in Brassica campestris. We identified 11 MIR159/319 genes in B. campestris, which produced five mature sequences. After analyzing the precursor sequences and phylogenetic tree, we found that Bra-MIR159/319 have evolutionary conservatism. Furthermore, Bra-MIR159/319 show functional diversification after WGT, as indicated by their expression patterns and the cis-element in their promoter. GUS signal showed that Bra-MIR159a and Bra-MIR319c can be expressed in anther but in different development stages. In B. campestris, overexpressed MIR159a and MIR319c contribute to late anther development and promote pollen abortion. Moreover, Bra-MIR319c can partially assume the function of MIR319a in flower development.

Keywords

Brassica campestris WGT Bra-MIR159/319 Evolution Functional diversification Pollen development 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31772311).

Author contributions

Ziwei Hu designed all the experiments. Xiuping She and Xun Xiang took part in this work and the manuscript modification. Jiaoshu Cao is the corresponding author.

Supplementary material

11103_2019_920_MOESM1_ESM.doc (5.2 mb)
Supplementary material 1 (DOC 5373 kb)
11103_2019_920_MOESM2_ESM.xls (27 kb)
Supplementary material 2 (XLS 27 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Ziwei Hu
    • 1
  • Xiuping Shen
    • 1
  • Xun Xiang
    • 1
    • 2
    • 3
  • Jiashu Cao
    • 1
    • 2
    • 3
    Email author
  1. 1.Laboratory of Cell and Molecular Biology, Institute of Vegetable ScienceZhejiang UniversityHangzhouChina
  2. 2.Key Laboratory of Horticultural Plant Growth, Development and Quality ImprovementMinistry of AgricultureHangzhouChina
  3. 3.Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative BiologyHangzhouChina

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