Proteomic analysis of the similarities and differences of soil drought and polyethylene glycol stress responses in wheat (Triticum aestivum L.)
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Our results reveal both soil drought and PEG can enhance malate, glutathione and ascorbate metabolism, and proline biosynthesis, whereas soil drought induced these metabolic pathways to a greater degree than PEG.
Polyethylene glycol (PEG) is widely used to simulate osmotic stress, but little is known about the different responses of wheat to PEG stress and soil drought. In this study, isobaric tags for relative quantification (iTRAQ)-based proteomic techniques were used to determine both the proteomic and physiological responses of wheat seedlings to soil drought and PEG. The results showed that photosynthetic rate, stomatal conductance, intercellular CO2 concentration, transpiration rate, maximum potential efficiency of PS II, leaf water content, relative electrolyte leakage, MDA content, and free proline content exhibited similar responses to soil drought and PEG. Approximately 15.8% of differential proteins were induced both by soil drought and PEG. Moreover, both soil drought and PEG inhibited carbon metabolism and the biosynthesis of some amino acids by altering the accumulation of glyceraldehyde-3-phosphate dehydrogenase, ribulose-bisphosphate carboxylase, and phosphoglycerate kinase, but they both enhanced the metabolism of malate, proline, glutathione, and ascorbate by increasing the accumulation of key enzymes including malate dehydrogenase, monodehydroascorbate reductase, pyrroline-5-carboxylate dehydrogenase, pyrroline-5-carboxylate synthetase, ascorbate peroxidase, glutathione peroxidase, and glutathione S-transferase. Notably, the latter five of these enzymes were found to be more sensitive to soil drought. In addition, polyamine biosynthesis was specifically induced by increased gene expression and protein accumulation of polyamine oxidase and spermidine synthase under PEG stress, whereas fructose-bisphosphate aldolase and arginase were induced by soil drought. Therefore, present results suggest that PEG is an effective method to simulate drought stress, but the key proteins related to the metabolism of malate, glutathione, ascorbate, proline, and polyamine need to be confirmed under soil drought.
KeywordsWater deficit Osmotic stress Proline Glutathione Ascorbate Polyamine
Differentially accumulated protein
Gene ontology annotation
Reactive oxygen species
YX thanks The National Key Basic Research Program, China (2017YFD0100706), Protection and Utilization of Germplasm Resources of Shaanxi Province, China (20171010000004), and Agriculture Technology Demonstration Project of Yangling, China (2017-TS-20) for financial support.
YX designed and directed this study as well as drafted and revised the manuscript. GC and YZ performed the experiments and analyzed the data as well as drafted and revised the manuscript. MC and JZ conducted the physiological and stress parameters determination. KX measured the gene expression of all selected proteins. FS, CZ and SL improved the data analysis and revised the manuscript.
- Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373–399. https://doi.org/10.1146/annurev.arplant.55.031903.141701 CrossRefGoogle Scholar
- Ashoub A, Baeumlisberger M, Neupaertl M, Karas M, Brüggemann W (2015) Characterization of common and distinctive adjustments of wild barley leaf proteome under drought acclimation, heat stress and their combination. Plant Mol Biol 87:459–471. https://doi.org/10.1007/s11103-015-0291-4 CrossRefGoogle Scholar
- Cheng L, Wang Y, He Q, Li H, Zhang X, Zhang F (2016) Comparative proteomics illustrates the complexity of drought resistance mechanisms in two wheat (Triticum aestivum L.) cultivars under dehydration and rehydration. BMC Plant Biol 16:188. https://doi.org/10.1186/s12870-016-0871-8 CrossRefGoogle Scholar
- Delauney AJ, Verma DPS (1993) Proline biosynthesis and osmoregulation in plants. Plant J 4:215–223. https://doi.org/10.1046/j.1365-313X.1993.04020215.x CrossRefGoogle Scholar
- Flexas J, Ribas‐Carbó M, Bota J, Galmés J, Henkle M, Martínez‐Cañellas S, Medrano H (2006) Decreased Rubisco activity during water stress is not induced by decreased relative water content but related to conditions of low stomatal conductance and chloroplast CO2 concentration. New Phytol 172:73–82. https://doi.org/10.1111/j.1469-8137.2006.01794.x
- He JX, An LZ, Lin HH, Liang HG (1999) Evidence for transcriptional and post-transcriptional control of protein synthesis in water-stressed wheat leaves: a quantitative analysis of messenger and ribosomal RNA. J Plant Physiol 155:63–69. https://doi.org/10.1016/S0176-1617(99)80141-2 CrossRefGoogle Scholar
- Hossain MA, Asada K (1984) Inactivation of ascorbate peroxidase in spinach chloroplasts on dark addition of hydrogen peroxide: its protection by ascorbate. Plant Cell Physiol 25:1285–1295. https://doi.org/10.1093/oxfordjournals.pcp.a076837 Google Scholar
- Kosová K, Urban MO, Vítámvás P, Prášil IT (2016) Drought stress response in common wheat, durum wheat, and barley: transcriptomics, proteomics, metabolomics, physiology, and breeding for an enhanced drought tolerance. In: Hossain MA, Wani SH, Bhattacharjee S, Burritt DJ, Tran L-SP (eds) Drought stress tolerance in plants, vol 2: molecular and genetic perspectives. Springer, Cham, pp 277–314 https://doi.org/10.1007/978-3-319-32423-4_11
- Li N, Zhang S, Liang Y, Qi Y, Chen J, Zhu W, Zhang L (2017) Label-free quantitative proteomic analysis of drought stress-responsive late embryogenesis abundant proteins in the seedling leaves of two wheat (Triticum aestivum L.) genotypes. J Proteomics 172:122–142. https://doi.org/10.1016/j.jprot.2017.09.016 CrossRefGoogle Scholar
- Liu H, Sultan MARF, Liu XL, Zhang J, Yu F, Zhao HX (2015) Physiological and comparative proteomic analysis reveals different drought responses in roots and leaves of drought-tolerant wild wheat (Triticum boeoticum). PLOS ONE 10(4):e0121852Google Scholar
- Miyake C, Asada K (1992) Thylakoid-bound ascorbate peroxidase in spinach chloroplasts and photoreduction of its primary oxidation product monodehydroascorbate radicals in thylakoids. Plant Cell Physiol 33:541–553. https://doi.org/10.1093/oxfordjournals.pcp.a078288 Google Scholar
- Nahar K, Hasanuzzaman M, Fujita M (2016) Roles of osmolytes in plant adaptation to drought and salinity. In: Iqbal N, Nazar R, Khan NA (eds) Osmolytes and plants acclimation to changing environment: emerging omics technologies. Springer, New Delhi, pp 37–68. https://doi.org/10.1007/978-81-322-2616-1_4
- Parida AK, Dagaonkar VS, Phalak MS, Aurangabadkar LPJAPP (2008) Differential responses of the enzymes involved in proline biosynthesis and degradation in drought tolerant and sensitive cotton genotypes during drought stress and recovery. Acta Physiol Plant 30:619–627. https://doi.org/10.1007/s11738-008-0157-3 CrossRefGoogle Scholar
- Peremarti A, Mare C, Aprile A, Roncaglia E, Cattivelli L, Villegas D, Royo C (2014) Transcriptomic and proteomic analyses of a pale-green durum wheat mutant shows variations in photosystem components and metabolic deficiencies under drought stress. BMC Genomics 15:125. https://doi.org/10.1186/1471-2164-15-125 CrossRefGoogle Scholar
- Samarah NH (2016) Understanding how plants respond to drought stress at the molecular and whole plant levels. In: Hossain MA, Wani SH, Bhattacharjee S, Burritt DJ, Tran L-SP (eds) Drought stress tolerance in plants, vol 2: molecular and genetic perspectives. Springer, Cham, pp 1–37. https://doi.org/10.1007/978-3-319-32423-4_1
- Tambussi EA, Bartoli CG, Beltrano J, Guiamet JJ, Araus JL (2000) Oxidative damage to thylakoid proteins in water-stressed leaves of wheat (Triticum aestivum). Physiol Plant 108:398–404. https://doi.org/10.1034/j.1399-3054.2000.108004398.x CrossRefGoogle Scholar