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Plant Molecular Biology

, Volume 100, Issue 4–5, pp 367–378 | Cite as

Recognition of S-RNases by an S locus F-box like protein and an S haplotype-specific F-box like protein in the Prunus-specific self-incompatibility system

  • Daiki MatsumotoEmail author
  • Ryutaro Tao
Article
  • 603 Downloads

Abstract

Key message

S-RNase was demonstrated to be predominantly recognized by an S locus F-box-like protein and an S haplotype-specific F-box-like protein in compatible pollen tubes of sweet cherry.

Abstract

Self-incompatibility (SI) is a reproductive barrier that rejects self-pollen and inhibits self-fertilization to promote outcrossing. In Solanaceae and Rosaceae, S-RNase-based gametophytic SI (GSI) comprises S-RNase and F-box protein(s) as the pistil and pollen S determinants, respectively. Compatible pollen tubes are assumed to detoxify the internalized cytotoxic S-RNases to maintain growth. S-RNase detoxification is conducted by the Skp1-cullin1-F-box protein complex (SCF) formed by pollen S determinants, S locus F-box proteins (SLFs), in Solanaceae. In Prunus, the general inhibitor (GI), but not pollen S determinant S haplotype-specific F-box protein (SFB), is hypothesized to detoxify S-RNases. Recently, SLF-like proteins 1–3 (SLFL1–3) were suggested as GI candidates, although it is still possible that other proteins function predominantly in GI. To identify the other GI candidates, we isolated four other pollen-expressed SLFL and SFB-like (SFBL) proteins PavSLFL6, PavSLFL7A, PavSFBL1, and PavSFBL2 in sweet cherry. Binding assays with four PavS-RNases indicated that PavSFBL2 bound to PavS1, 6-RNase while the others bound to nothing. PavSFBL2 was confirmed to form an SCF complex in vitro. A co-immunoprecipitation assay using the recombinant PavS6-RNase as bait against pollen extracts and a mass spectrometry analysis identified the SCF complex components of PavSLFLs and PavSFBL2, M-locus-encoded glutathione S-transferase (MGST), DnaJ-like protein, and other minor proteins. These results suggest that SLFLs and SFBLs could act as predominant GIs in Prunus-specific S-RNase-based GSI.

Keywords

General inhibitor Prunus Self-incompatibility SFBL SLFL S-RNase 

Notes

Acknowledgements

This work was supported by a Grant-in-Aid (No. 16H06184) for Young Scientists (A) from the Japan Society for the Promotion of Science to D. M.

Author contributions

D. M. designed and conducted the experiments and drafted the manuscript. Both authors edited the manuscript and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11103_2019_860_MOESM1_ESM.eps (1019 kb)
Supplementary material 1 (EPS 1019 kb). Supplementary Fig. 1 Expression analysis of SLF-like and SFB-like homologs in Japanese apricot cv. Nanko pollen: expression levels were calculated from transcriptome data previously reported (DRR002283; Akagi et al. 2016) and expressed as FPKM
11103_2019_860_MOESM2_ESM.eps (1.4 mb)
Supplementary material 2 (EPS 1384 kb). Supplementary Fig. 2 The alignment of the N-terminal regions of the deduced amino-acid sequences of SLFL7 homologs of sweet cherry, peach and Japanese apricot:The MUSCLE program implemented in MEGA ver. 6.0 (Tamura et al. 2013) was used to generate the alignment. Amino acids shared over 75% among the homologs are shaded. The dashed line represents the region corresponding to the F-box motif in other Prunus SLFLs, SFB and SFBLs. Asterisks represent the amino-acid substitution in PavSLFL7A, which was shown to impair its interaction with PavSSK1
11103_2019_860_MOESM3_ESM.eps (873 kb)
Supplementary material 3 (EPS 873 kb). Supplementary Fig. 3 The GST-pulldown assay of PavCul1A and Prunus avium Rbx1-like protein (XP_021828912.1). 3 × HA-tagged PavCul1A and GST-fused Rbx1-like protein were co-expressed in the cell-free system. The protein complexes bound to the glutathione sepharose beads were detected by the immunoblot

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Laboratory of Pomology, Faculty of AgricultureYamagata UniversityTsuruokaJapan
  2. 2.Graduate School of AgricultureKyoto UniversityKyotoJapan

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