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PpCBFs selectively regulate PpDAMs and contribute to the pear bud endodormancy process

  • Jianzhao Li
  • Xinhui Yan
  • Qinsong Yang
  • Yunjing Ma
  • Bo Yang
  • Juan Tian
  • Yuanwen TengEmail author
  • Songling BaiEmail author
Article

Abstract

Key message

PpCBF2 directly binds to the promoters of PpCBF3 and PpCBF4 to activate their expressions and selectively regulates PpDAMs during the leaf bud endodormancy process of ‘Wonhwang’ pear (Pyrus pyrifolia).

Abstract

Endodormancy is critical for temperate plant survival under freezing winter conditions, and low temperature is a vital environmental factor in endodormancy regulation. A C-repeat binding factor (CBF) has been found to regulate important DAM transcription factors during endodormancy in pear (Pyrus pyrifolia). In this study, we analyzed the regulation of pear DAM genes by CBFs in further detail. Four CBF and three DAM genes were identified in the pear cultivar ‘Wonhwang’. Under natural conditions, PpDAM1 expression decreased from the start of chilling accumulation, while the other two DAM and three CBF genes peaked during endodormancy release. Under chilling treatment, the expressions of PpDAM1, PpDAM2 and PpCBF1 genes were similar to those under natural conditions. Different biochemical methods revealed that PpCBF2/4 can bind to the promoter of PpDAM1 and activate its expression and that PpCBF1/4 can activate PpDAM3. Interestingly, we found that PpCBF2 can activate PpCBF3/4 transcription by directly binding to their promoters. The ICE-CBF regulon is conserved in some plants; three ICE genes were identified in pear, but their expressions did not obviously change under natural and artificial chilling conditions. On the contrary, the selective transcriptional induction of PpCBFs by PpICE1s was observed in a dual-luciferase assay. Considering all these results, we propose that the PpCBF1-PpDAM2 regulon mainly responds to low temperature during endodormancy regulation, with further post-translational regulation by PpICE3. Our results provide basic information on CBF genes functional redundancy and differentiation and demonstrate that the CBF-DAM signaling pathway is involved in the pear bud endodormancy process.

Keywords

Pear Endodormancy CBF DAM ICE 

Abbreviations

AbA

Aureobasidin A

CBF

C-repeat binding factor

COR

Cold-regulated

CRT/DRE

C-repeat/dehydration responsive element

CU

Chilling unit

DAM

Dormancy associated MADS-box gene

LUC/REN

Firefly luciferase/Renilla luciferase

qRT- PCR

Quantitative real-time PCR

ICE

Inducer of CBF expression

Y1H

Yeast one-hybrid

Notes

Author contributions

SB and YT conceived and supervised the project; JL, and SB designed the experiments; JL and XY performed most of the experiments; QY, YM and BY carried out some of the experiments; JT collected pear bud samples; JL, YT and SB analyzed the data and wrote the manuscript. All authors read and approved the final manuscript.

Funding

This research was supported by National Natural Science Foundation of China (No. 31501736); Earmarked Fund for China Agriculture Research System (CARS-28) and the Fundamental Research Funds for the Central Universities (2018QNA6022).

Compliance with ethical standards

Conflict of interest

The authors declare there are no conflicts of interest.

Supplementary material

11103_2019_837_MOESM1_ESM.docx (1.6 mb)
Supplementary material 1 (DOCX 1601 KB)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of HorticultureZhejiang UniversityHangzhouPeople’s Republic of China
  2. 2.The Key Laboratory of Horticultural Plant Growth, Development and Quality ImprovementThe Ministry of Agriculture of ChinaHangzhouPeople’s Republic of China
  3. 3.Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural PlantsHangzhouPeople’s Republic of China
  4. 4.Dangshan County Agriculture CommitteeSuzhouPeople’s Republic of China

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