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Plant Molecular Biology

, Volume 99, Issue 1–2, pp 67–78 | Cite as

Ethylene response factors Pp4ERF24 and Pp12ERF96 regulate blue light-induced anthocyanin biosynthesis in ‘Red Zaosu’ pear fruits by interacting with MYB114

  • Junbei Ni
  • Songling Bai
  • Yuan Zhao
  • Minjie Qian
  • Ruiyan Tao
  • Lei Yin
  • Ling Gao
  • Yuanwen TengEmail author
Article

Abstract

Key message

Pp4ERF24 and Pp12ERF96 fine tune blue light-induced anthocyanin biosynthesis via interacting with PpMYB114 and promoting the interaction between PpMYB114 and PpbHLH3, which enhances the expression of PpMYB114-induced PpUFGT.

Abstract

The red coloration of pear fruit is attributed to anthocyanin accumulation, which is transcriptionally regulated by the MYB-bHLH-WD40 complex. A number of ethylene response factors (ERF) have been identified to regulate anthocyanin biosynthesis in different plants. In pear, several ERF transcription factor genes were identified to be potentially involved in the light-induced anthocyanin biosynthesis according to transcriptome data. But the molecular mechanism of these ERFs underlying the regulation of anthocyanin accumulation is unknown. In this study, exposure of ‘Red Zaosu’ pear, a mutant of ‘Zaosu’ pear, to blue light significantly induced the anthocyanin accumulation by increasing the expression levels of anthocyanin biosynthetic genes. Gene expression analysis confirmed that the expression of Pp4ERF24 and Pp12ERF96 genes were up-regulated in the process of blue light-induced anthocyanin biosynthesis. Yeast two-hybrid and bimolecular fluorescence complementation assay revealed that Pp4ERF24 and Pp12ERF96 interacted with PpMYB114, but not with PpMYB10. Bimolecular fluorescence complementation assay demonstrated that the interaction between these two ERFs and PpMYB114 enhanced the interaction between PpMYB114 and PpbHLH3. Further analysis by dual luciferase assay verified that these two ERFs increased the up-regulation of PpMYB114-mediated PpUFGT expression. Furthermore, co-transformation of Pp12ERF96 with PpMYB114 and PpbHLH3 in tobacco leaves led to enhanced anthocyanin accumulation. Transient overexpression of Pp4ERF24 or Pp12ERF96 alone in ‘Red Zaosu’ pear fruit also induced anthocyanin biosynthesis in pear peel. Our findings provide insights into a mechanism involving the synergistic interaction of ERFs with PpMYB114 to regulate light-dependent coloration and anthocyanin biosynthesis in pear fruits.

Keywords

Anthocyanin Pear (PyrusERF MYB Blue light 

Abbreviations

ERF

Ethylene response factor

UFGT

UDP-glucose: flavonoid 3-glucosyltransferase

EBG

Early biosynthetic gene

LBG

Late biosynthetic gene

PAL

Phenylalanine ammonia lyase

CHS

Chalcone synthase

CHI

Chalcone isomerase

F3′H

Flavanone 3′-hydroxylase

DFR

Dihydroflavonol 4-reductase

ANS

Anthocyanin synthase

bHLH

Basic helix-loop-helix

DAFB

Days after full bloom

Notes

Acknowledgements

This study was supported by the National Natural Science Foundation of China (Grant Nos. 31471852 to YT and 31772272 to SB) and the Earmarked Fund for China Agriculture Research System (CARS-28).

Author contributions

JN, SB, and YT conceived and planned the study. RT and LY helped collect samples and extracted the total RNA. JN and YZ completed the qPCR, vector construction, and bioinformatics analysis. JN and LG conducted the Y2H, BiFC, dual luciferase, firefly luciferase complementation imaging assays and the transient transformation assay of gene function in tobacco leaves and ‘Red Zaosu’ fruits. JN, SB, MQ and YT wrote the manuscript. All of the authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interest.

Supplementary material

11103_2018_802_MOESM1_ESM.docx (15 kb)
Supplementary material 1 (DOCX 14 KB)
11103_2018_802_MOESM2_ESM.docx (14 kb)
Supplementary material 2 (DOCX 13 KB)
11103_2018_802_MOESM3_ESM.docx (15 kb)
Supplementary material 3 (DOCX 14 KB)
11103_2018_802_MOESM4_ESM.pdf (714 kb)
(A) Phylogenetic analysis of ERF proteins from ‘Red Zaosu’ pear (PpERF) and Prunus persica (PpeERF). (B) Pp4ERF24, Pp12ERF96, Pp12ERF100, and Pp1ERF1 expression patterns based on ‘Meirensu’ transcriptome data. Supplementary material 4 (PDF 721 KB)
11103_2018_802_MOESM5_ESM.pdf (4.4 mb)
(A) A yeast two-hybrid (Y2H) assay revealed that Pp4ERF24, Pp12ERF96, Pp12ERF100, and Pp1ERF1 did not interact with PpMYB10. The pGADT7-T and pGBKT7-53 vectors were used as the positive control, while the AD and MYB10-BD combination served as the negative control. Blue plaques indicated an interaction between two proteins. The basal AbA concentration was 200 ng ml−1. (B) A Y2H assay confirmed that PpMYB114, Pp12ERF96, Pp12ERF100, and Pp1ERF1 underwent an auto-activation. The BD vector was used as the negative control. Supplementary material 5 (PDF 4485 KB)
11103_2018_802_MOESM6_ESM.pdf (722 kb)
Analysis of the Pp4ERF24 and Pp12ERF96 promoters revealed several light-responsive elements. Supplementary material 6 (PDF 713 KB)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Junbei Ni
    • 1
    • 2
    • 3
  • Songling Bai
    • 1
    • 2
    • 3
  • Yuan Zhao
    • 1
    • 2
    • 3
  • Minjie Qian
    • 4
  • Ruiyan Tao
    • 1
    • 2
    • 3
  • Lei Yin
    • 1
    • 2
    • 3
  • Ling Gao
    • 5
  • Yuanwen Teng
    • 1
    • 2
    • 3
    Email author
  1. 1.Department of HorticultureZhejiang UniversityHangzhouPeople’s Republic of China
  2. 2.The Key Laboratory of Horticultural Plant Growth, Development and Quality ImprovementThe Ministry of Agriculture of ChinaHangzhouPeople’s Republic of China
  3. 3.Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural PlantsHangzhouPeople’s Republic of China
  4. 4.School of Science and TechnologyÖrebro UniversityÖrebroSweden
  5. 5.ACON Biotech (Hangzhou) Co., Ltd.HangzhouPeople’s Republic of China

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