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Pharmaceutical Research

, 36:104 | Cite as

The Natural Product Eugenol Is an Inhibitor of the Ebola Virus In Vitro

  • Thomas Lane
  • Manu Anantpadma
  • Joel S. Freundlich
  • Robert A. Davey
  • Peter B. Madrid
  • Sean EkinsEmail author
Commentary

Abstract

Purpose

Since the 2014 Ebola virus (EBOV) outbreak in West Africa there has been considerable effort towards developing drugs to treat Ebola virus disease and yet to date there is no FDA approved treatment. This is important as at the time of writing this manuscript there is an ongoing outbreak in the Democratic Republic of the Congo which has killed over 1000.

Methods

We have evaluated a small number of natural products, some of which had shown antiviral activity against other pathogens. This is exemplified with eugenol, which is found in high concentrations in multiple essential oils, and has shown antiviral activity against feline calicivirus, tomato yellow leaf curl virus, Influenza A virus, Herpes Simplex virus type 1 and 2, and four airborne phages.

Results

Four compounds possessed EC50 values less than or equal to 11 μM. Of these, eugenol, had an EC50 of 1.3 μM against EBOV and is present in several plants including clove, cinnamon, basil and bay. Eugenol is much smaller and structurally unlike any compound that has been previously identified as an inhibitor of EBOV, therefore it may provide new mechanistic insights.

Conclusion

This compound is readily accessible in bulk quantities, is inexpensive, and has a long history of human consumption, which endorses the idea for further assessment as an antiviral therapeutic. This work also suggests that a more exhaustive assessment of natural product libraries against EBOV and other viruses is warranted to improve our ability to identify compounds that are so distinct from FDA approved drugs.

Key words

antiviral drug discovery ebola eugenol p-anisaldehyde 

Notes

Acknowledgments and Disclosures

Ms. Kimberley M. Zorn, Dr. Mary A. Lingerfelt and Dr. Alex M. Clark, are kindly acknowledged for their assistance. T.L., and S.E. work for Collaborations Pharmaceuticals, Inc.

Funding

SE kindly acknowledges NIH funding: R21TR001718 from NCATS (PI – Sean Ekins).

Supplementary material

11095_2019_2629_MOESM1_ESM.docx (398 kb)
ESM 1 (DOCX 397 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Collaborations Pharmaceuticals, Inc.North CarolinaUSA
  2. 2.Texas Biomedical Research InstituteSan AntonioUSA
  3. 3.National Emerging Infectious Diseases LaboratoriesBoston UniversityBostonUSA
  4. 4.Departments of Pharmacology & Physiology and Medicine, Center for Emerging and Reemerging PathogensRutgers University – New Jersey Medical SchoolSouth OrangeUSA
  5. 5.Biosciences DivisionSRI InternationalCaliforniaUSA

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