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Cefquinome-Loaded Microsphere Formulations in Protection against Pneumonia with Klebsiella pneumonia Infection and Inflammatory Response in Rats

  • Shaoqi Qu
  • Cunchun Dai
  • Fenfang Yang
  • Tingting Huang
  • Zhihui HaoEmail author
  • Qihe Tang
  • Haixia Wang
  • Yanping Zhang
Research Paper
  • 166 Downloads

Abstract

Purpose

This study aimed to compare in vivo activity between cefquinome (CEQ)-loaded poly lactic-co-glycolic acid (PLGA) microspheres (CEQ-PLGA-MS) and CEQ injection (CEQ-INJ) against Klebsiella pneumonia in a rat lung infection model.

Methods

Forty-eight rats were divided into control group (sham operated without infection and drug treatment), Klebsiella pneumonia model group (KPD + Saline), CEQ-PLGA-MS and CEQ-INJ therapy groups (KPD + CEQ-PLGA-MS and KPD + INJ, respectively). In the KPD + Saline group, rats were infected with Klebsiella pneumonia ATCC 10031. In the KPD + CEQ-PLGA-MS and KPD + INJ groups, infected rats were intravenously injected with 12.5 mg/kg body weight CEQ-PLGA-MS and CEQ-INJ, respectively.

Results

Compared to CEQ-INJ treatment group, CEQ-PLGA-MS treatment further decreased the number of bacteria colonies (decreased to 1.94 lg CFU/g) in lung tissues and the levels of inflammatory cytokine including tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-4 (p < 0.05 or p < 0.01) in bronchoalveolar lavage fluid at 48 h. Consistently, a significant decreases of scores of inflammation severity were showed at 48 h in the KPD + CEQ-PLGA-MS treatment group, compared to the KPD + CEQ-INJ treatment group.

Conclusion

Our results reveal that CEQ-PLGA-MS has the better therapeutic effect than CEQ-INJ for Klebsiella pneumonia lung infections in rats. The vehicle of CEQ-PLGA-MS as the promising alternatives to control the lung infections with the important pathogens.

Keywords

cefquinome inflammation Klebsiella pneumonia pharmacodynamics poly lactic-co-glycolic acid microspheres 

Abbreviations

BALF

Bronchoalveolar lavage fluid

CEQ

Cefquinome

CEQ-INJ

CEQ injection

CEQ-PLGA-MS

CEQ-loaded PLGA microspheres

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

IL

Interleukin

i.v.

Intravenously

K. pneumonia

Klebsiella pneumonia

PD

Pharmacodynamic

PLGA

Poly lactic-co-glycolic acid

qRT-PCR

Quantitative reverse transcription-PCR

RPMI

Roswell Park Memorial Institute

SD

Standard deviation

TNF

Tumor necrosis factor

Notes

ACKNOWLEDGMENTS AND DISCLOSURES

This work was supported by the national key research and development plan (NO. 2016YFD0501309) and startup and innovation leader talent plan of Qingdao 15–10–3-15-(41)-zch. The authors declare no conflicts of interest.

Supplementary material

11095_2019_2614_MOESM1_ESM.pdf (14 kb)
ESM 1 (PDF 14.1 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Shaoqi Qu
    • 1
    • 2
  • Cunchun Dai
    • 1
    • 2
  • Fenfang Yang
    • 1
    • 2
  • Tingting Huang
    • 1
    • 2
  • Zhihui Hao
    • 1
    • 2
    Email author
  • Qihe Tang
    • 1
    • 2
  • Haixia Wang
    • 1
    • 2
  • Yanping Zhang
    • 1
    • 2
  1. 1.College of Chemistry and Pharmaceutical SciencesQingdao Agricultural UniversityQingdaoChina
  2. 2.National-Local Joint Engineering Laboratory of Agricultural Bio-pharmaceutical TechnologyQingdaoChina

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