Evaluation of Wound Healing Potential of β-Chitin Hydrogel/Nano Zinc Oxide Composite Bandage
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β-chitin hydrogel/nZnO composite bandage was fabricated and evaluated in detail as an alternative to existing bandages.
β-chitin hydrogel was synthesized by dissolving β-chitin powder in Methanol/CaCl2 solvent, followed by the addition of distilled water. ZnO nanoparticles were added to the β-chitin hydrogel and stirred for homogenized distribution. The resultant slurry was frozen at 0°C for 12 h. The frozen samples were lyophilized for 24 h to obtain porous composite bandages.
The bandages showed controlled swelling and degradation. The composite bandages showed blood clotting ability as well as platelet activation, which was higher when compared to the control. The antibacterial activity of the bandages were proven against Staphylococcus aureus (S. aureus) and Escherichia coli (E.coli). Cytocompatibility of the composite bandages were assessed using human dermal fibroblast cells (HDF) and these cells on the composite bandages were viable similar to the Kaltostat control bandages and bare β-chitin hydrogel based bandages. The viability was reduced to 50–60% in bandages with higher concentration of zinc oxide nanoparticles (nZnO) and showed 80–90% viability with lower concentration of nZnO. In vivo evaluation in Sprague Dawley rats (S.D. rats) showed faster healing and higher collagen deposition ability of composite bandages when compared to the control.
The prepared bandages can be used on various types of infected wounds with large volume of exudates.
KEY WORDSantibacterial bandage nano ZnO wound healing β-chitin hydrogel
ACKNOWLEDGMENTS AND DISCLOSURES
The authors acknowledge Department of Biotechnology (DBT), India, for the financial support under a grant (BT/PR13885/MED/32/145/2010 dated 03-01-2011). We are also grateful to Nanomission, Department of Science and Technology, India, which supported this work, under a grant of the Nanoscience and Nanotechnology Initiative program. The author “R. Jayakumar” is grateful to SERC Division, Department of Science and Technology (DST), India, for providing the fund under the scheme of “Fast Track Scheme for Young Investigators” (Ref. No. SR/FT/CS-005/2008). Raja Biswas acknowledges Ramalingaswami Fellowship, Department of Biotechnology, India, for the financial support. P T Sudheesh Kumar acknowledges the Council of Scientific and Industrial Research, India for the Senior Research Fellowship (Award No. 9/963 (0011) 2K11- EMR-1).We are also grateful to Mr. Sajin P. Ravi for his help in SEM analysis. We acknowledge K. S. Sarath for his help in confocal imaging. We are grateful to Dr. P. Reshmi, Dr. A.K.K. Unni, Sajith, Sunil and Sunitha for their help during in vivo study. We thank Amrita Centre for Nanosciences and Molecular Medicine for the infrastructure support.
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