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Pharmaceutical Research

, Volume 30, Issue 2, pp 523–537 | Cite as

Evaluation of Wound Healing Potential of β-Chitin Hydrogel/Nano Zinc Oxide Composite Bandage

  • Sudheesh Kumar P. T.
  • Vinoth-Kumar Lakshmanan
  • Mincy Raj
  • Raja Biswas
  • Tamura Hiroshi
  • Shantikumar V. Nair
  • Rangasamy JayakumarEmail author
Research Paper

ABSTRACT

Purpose

β-chitin hydrogel/nZnO composite bandage was fabricated and evaluated in detail as an alternative to existing bandages.

Methods

β-chitin hydrogel was synthesized by dissolving β-chitin powder in Methanol/CaCl2 solvent, followed by the addition of distilled water. ZnO nanoparticles were added to the β-chitin hydrogel and stirred for homogenized distribution. The resultant slurry was frozen at 0°C for 12 h. The frozen samples were lyophilized for 24 h to obtain porous composite bandages.

Results

The bandages showed controlled swelling and degradation. The composite bandages showed blood clotting ability as well as platelet activation, which was higher when compared to the control. The antibacterial activity of the bandages were proven against Staphylococcus aureus (S. aureus) and Escherichia coli (E.coli). Cytocompatibility of the composite bandages were assessed using human dermal fibroblast cells (HDF) and these cells on the composite bandages were viable similar to the Kaltostat control bandages and bare β-chitin hydrogel based bandages. The viability was reduced to 50–60% in bandages with higher concentration of zinc oxide nanoparticles (nZnO) and showed 80–90% viability with lower concentration of nZnO. In vivo evaluation in Sprague Dawley rats (S.D. rats) showed faster healing and higher collagen deposition ability of composite bandages when compared to the control.

Conclusions

The prepared bandages can be used on various types of infected wounds with large volume of exudates.

KEY WORDS

antibacterial bandage nano ZnO wound healing β-chitin hydrogel 

Notes

ACKNOWLEDGMENTS AND DISCLOSURES

The authors acknowledge Department of Biotechnology (DBT), India, for the financial support under a grant (BT/PR13885/MED/32/145/2010 dated 03-01-2011). We are also grateful to Nanomission, Department of Science and Technology, India, which supported this work, under a grant of the Nanoscience and Nanotechnology Initiative program. The author “R. Jayakumar” is grateful to SERC Division, Department of Science and Technology (DST), India, for providing the fund under the scheme of “Fast Track Scheme for Young Investigators” (Ref. No. SR/FT/CS-005/2008). Raja Biswas acknowledges Ramalingaswami Fellowship, Department of Biotechnology, India, for the financial support. P T Sudheesh Kumar acknowledges the Council of Scientific and Industrial Research, India for the Senior Research Fellowship (Award No. 9/963 (0011) 2K11- EMR-1).We are also grateful to Mr. Sajin P. Ravi for his help in SEM analysis. We acknowledge K. S. Sarath for his help in confocal imaging. We are grateful to Dr. P. Reshmi, Dr. A.K.K. Unni, Sajith, Sunil and Sunitha for their help during in vivo study. We thank Amrita Centre for Nanosciences and Molecular Medicine for the infrastructure support.

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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Sudheesh Kumar P. T.
    • 1
  • Vinoth-Kumar Lakshmanan
    • 1
  • Mincy Raj
    • 1
  • Raja Biswas
    • 1
  • Tamura Hiroshi
    • 2
  • Shantikumar V. Nair
    • 1
  • Rangasamy Jayakumar
    • 1
    Email author
  1. 1.Amrita Centre for Nanosciences and Molecular Medicine Amrita Institute of Medical Sciences and Research CentreAmrita Vishwa Vidyapeetham UniversityKochiIndia
  2. 2.Faculty of Chemistry, Materials and BioengineeringKansai UniversityOsakaJapan

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