Water-Soluble Anionic C60-Fullerene Derivatives as Antidotes for HG(II) Ions in Tests on Escherichia Coli Cells
Three water-soluble C60-fullerene derivatives functionalized by anionic adducts of various chemical compositions and structures were prepared in highly specific reactions of chlorofullerene C60Cl6. Hybrid metal-organic complexes formed and aggregated in several instances in aqueous solution in the presence of stoichiometric (1:1 by moles) amounts of synthesized C60-fullerene derivatives and Hg(II) ions. The bioavailability of Hg(II) ions for Escherichia coli cells was reduced by such a reaction and manifested as decreased toxicity for strain E. coli K12 TG1 pF1 or weakened protective reactions of strain E. coli K12 MG1655 pMerA'::lux. The most pronounced protective effect was recorded for the C60-fullerene derivative functionalized by S-containing adducts of general formula –S(CH2)10CO2K with antidote activity comparable with that of the pharmacopoeial preparation Unithiol.
KeywordsC60-fullerene HgCl2 bioavailability Escherichia coli
The studies were financially supported by RFBR Grant No. 16-53-52030 and the Ministry of Education and Science of the RF (No. 0089-2019-0010) (synthesis of fullerene derivatives) and State Task for Research No. 0761-2019-0005 (bioavailability studies of Hg ions after reacting with fullerene derivatives).
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