Cold Atmospheric Pressure Plasma Can Induce Adaptive Response in Pea Seeds
- 12 Downloads
This study investigated the effect of cold atmospheric pressure air plasma pre-treatment on pea (Pisum sativum L.) seeds. The aim of our study was to verify the plasma impact on DNA damage and the induction of positive adaptive response in pea seedlings. The pea seeds were treated by plasma at the exposure times ranging from 60 to 300 s. Plasma was generated using the coplanar type of dielectric surface barrier discharge. The main plasma properties were estimated by electrical and by optical emission spectroscopy measurements. The DNA damage was evaluated by the alkaline comet assay. The adaptive response on seedlings was tested using the toxic concentration of zeocin. The positive effect of plasma pre-treatment and the reduction of DNA damage of pea seedlings in comparison to control samples without plasma was observed at all exposure times used. The strongest repairing effect was observed at the plasma treatment time in the range from 120 to 240 s. These results verify the safety of plasma application in agriculture at germination and growth enhancement and the existence of a plasma induced adaptive response.
KeywordsCold atmospheric pressure plasma Pea seeds Adaptive response Comet assay Zeocin
This work was supported by the Slovak Research and Development Agency under the Contract No. APVV-16-0216 and by Project VEGA 1/0410/18.
- 3.Lichtenthaler HK (1998) The stress concept in plants: an introduction. Ann N Y Acad Sci 851:187–198. https://doi.org/10.1111/j.1749-6632.1998.tb08993.x CrossRefPubMedGoogle Scholar
- 7.Asad NR, Asad LMBO, Silva AB et al (1998) Hydrogen peroxide induces protection against lethal effects of cumene hydroperoxide in Escherichia coli cells: an Ahp dependent and OxyR independent system? Mutat Res DNA Repair 407:253–259. https://doi.org/10.1016/S0921-8777(98)00010-X CrossRefPubMedGoogle Scholar
- 14.Santa-Gonzalez GA, Gomez-Molina A, Arcos-Burgos M et al (2016) Distinctive adaptive response to repeated exposure to hydrogen peroxide associated with upregulation of DNA repair genes and cell cycle arrest. Redox Biol 9:124–133. https://doi.org/10.1016/j.redox.2016.07.004 CrossRefPubMedPubMedCentralGoogle Scholar
- 18.Gateva S, Jovtchev G, Stankov A (2015) Salvia extract can decrease DNA damage induced by Zeocin. Int J Pharma Med Biol Sci 4:1–10Google Scholar
- 19.Chankova SG, Dimova E, Dimitrova M, Bryant PE (2007) Induction of DNA double-strand breaks by zeocin in Chlamydomonas reinhardtii and the role of increased DNA double-strand breaks rejoining in the formation of an adaptive response. Radiat Environ Biophys 46:409–416. https://doi.org/10.1007/s00411-007-0123-2 CrossRefGoogle Scholar
- 28.Laux CO (2002) Radiation and nonequilibrium collisional-radiative models. In: Fletcher D, Charbonnier M, Sarma GSR, Magin T (eds) von Karman institute lecture series 2002–07, physico-chemical modeling of high enthalpy and plasma flows. Rhode-Saint-Genèse, BelgiumGoogle Scholar
- 36.Olvera-Carrillo Y, Campos F, Reyes JL et al (2010) Functional analysis of the group 4 late embryogenesis abundant proteins reveals their relevance in the adaptive response during water deficit in arabidopsis. Plant Physiol 154:373–390. https://doi.org/10.1104/pp.110.158964 CrossRefPubMedPubMedCentralGoogle Scholar