Hemoglobin as a Diagnosing Molecule for Biological Effects of Atmospheric-Pressure Plasma
The studies with proteins are necessary to understand the biological effects of atmospheric pressure plasma (APP). Among proteins, those with transient metal ions play key roles in many biological events and they are very sensitive to environmental redox states. Iron-containing hemoglobin (Hb) is investigated in this study, after APP treatments under two environmental gas conditions of pure N2 and N2 + O2 mixture. Monitoring the intensity change for absorption spectra could lead to a quantitative assessment of the effect of discharge plasma on Hb. Redox states of Hb are classified into five states including O2-bound Hb (oxy-Hb), deoxy-Hb, met-Hb, NO-bound Hb (NO-Hb), and hemichrome. Chemically generated reactive species and some scavengers are applied to understand the chemical reactions. Our experimental results confirm the complex chemical reactions of APP and suggest the possible use of Hb as a model protein for the visualization of APP biological effects.
KeywordsAtmospheric pressure plasma Hemoglobin Reactive oxygen species (ROS) Reactive nitrogen species (RNS) ROS/RNS scavengers
This research was supported by Leading Foreign Research Institute Recruitment Program through the National Research Foundation of Korea (NRF) funded by the Korea government (MSIP) (NRF-2016K1A4A3914113) and (NRF-2016R1A1A1A05005431). It was partly supported by the research grant of Kwangwoon University in 2016.
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Conflict of interest
The author declares they have no conflicting financial interests.
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