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Neurochemical Research

, Volume 44, Issue 9, pp 2113–2122 | Cite as

The Effect of SPTLC2 on Promoting Neuronal Apoptosis is Alleviated by MiR-124-3p Through TLR4 Signalling Pathway

  • Xinhong Su
  • Yuqin Ye
  • Yongxiang Yang
  • Kailiang Zhang
  • Wei Bai
  • Huijun Chen
  • Enming Kang
  • Chuiguang Kong
  • Xiaosheng HeEmail author
Original Paper
  • 51 Downloads

Abstract

To investigate the role and mechanism of microRNA-124-3p (miR-124-3p) and serine palmitoyltransferase long chain base subunit 2 (SPTLC2) in neuronal apoptosis induced by mechanical injury. Transient transfection was used to modify the expression of miR-124-3p and SPTLC2. After transfection, neuronal apoptosis was evaluated in an in vitro injury model of primary neurons using TUNEL staining and western blot. The correlation between miR-124-3p and SPTLC2 was identified through a dual luciferase reporter assay in HEK293 cells. A rescue experiment in primary neurons was performed to further confirm the result. To explore the downstream mechanisms, co-immunoprecipitation was performed to identify proteins that interact with SPTLC2 in toll-like receptor 4 (TLR4) signalling pathway. Subsequently, the relative expression levels of TLR4 pathway molecules were measured by western blot. Our results showed that increased miR-124-3p can inhibit neuronal apoptosis, which is opposite to the effect of SPTLC2. In addition, miR-124-3p was proved to negatively regulate SPTLC2 expression and suppress the apoptosis-promoting effect of SPTLC2 via the TLR4 signalling pathway.

Keywords

miR-124-3p SPTLC2 Apoptosis TLR4 

Notes

Funding

This study was supported by the Natural Science Foundation of China (No. 81471264).

Compliance with Ethical Standards

Conflict of interest

The authors have no conflicts of interest to disclose.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Xinhong Su
    • 1
  • Yuqin Ye
    • 2
  • Yongxiang Yang
    • 1
  • Kailiang Zhang
    • 3
  • Wei Bai
    • 1
  • Huijun Chen
    • 1
  • Enming Kang
    • 1
  • Chuiguang Kong
    • 1
  • Xiaosheng He
    • 1
    Email author
  1. 1.Department of NeurosurgeryXijing Hospital, Air Force Military Medical UniversityXi’anChina
  2. 2.Department of NeurosurgeryPLA 921rd HospitalChangshaChina
  3. 3.Department of Orthopedic Surgery, Orthopedic Oncology Institute of Chinese PLATangdu Hospital, Air Force Military Medical UniversityXi’anChina

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