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Neurochemical Research

, Volume 44, Issue 1, pp 133–146 | Cite as

Effects of Ethanol Exposure on the Neurochemical Profile of a Transgenic Mouse Model with Enhanced Glutamate Release Using In Vivo 1H MRS

  • Wen-Tung Wang
  • Phil Lee
  • Dongwei Hui
  • Elias K. Michaelis
  • In-Young ChoiEmail author
Original Paper
  • 46 Downloads

Abstract

Ethanol (EtOH) intake leads to modulation of glutamatergic transmission, which may contribute to ethanol intoxication, tolerance and dependence. To study metabolic responses to the hyper glutamatergic status at synapses during ethanol exposure, we used Glud1 transgenic (tg) mice that over-express the enzyme glutamate dehydrogenase in brain neurons and release excess glutamate (Glu) in synapses. We measured neurochemical changes in the hippocampus and striatum of tg and wild-type (wt) mice using proton magnetic resonance spectroscopy before and after the animals were fed with diets within which EtOH constituting up to 6.4% of total calories for 24 weeks. In the hippocampus, the EtOH diet led to significant increases in concentrations of EtOH, glutamine (Gln), Glu, phosphocholine (PCho), taurine, and Gln + Glu, when compared with their baseline concentrations. In the striatum, the EtOH diet led to significant increases in concentrations of GABA, Gln, Gln + Glu, and PCho. In general, neurochemical changes were more pronounced in the striatum than the hippocampus in both tg and wt mice. Overall neurochemical changes due to EtOH exposure were very similar in tg and wt mice. This study describes time courses of neurochemical profiles before and during chronic EtOH exposure, which can serve as a reference for future studies investigating ethanol-induced neurochemical changes.

Keywords

Glud1 transgenic mice Enhanced glutamate release Ethanol exposure Magnetic resonance spectroscopy 

Notes

Funding

This study was funded by National Institute on Aging (AG 12993, AG 035982), Kansas City Area Life Sciences Institute (KCALSI-07-13).

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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Hoglund Brain Imaging CenterUniversity of Kansas Medical CenterKansas CityUSA
  2. 2.Department of Molecular & Integrative PhysiologyUniversity of Kansas Medical CenterKansas CityUSA
  3. 3.Department of NeurologyUniversity of Kansas Medical CenterKansas CityUSA
  4. 4.Department of Pharmacology and ToxicologyUniversity of KansasLawrenceUSA

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