High-Dose Tanshinone IIA Suppresses Migration and Proliferation While Promoting Apoptosis of Astrocytoma Cells Via Notch-1 Pathway
- 92 Downloads
Malignant astrocytoma is the most common malignant tumor with strong invasion in the central nervous system. Tanshinone IIA is an effective compound to suppress cell proliferation and promote cell apoptosis. However, there is little research about the role of tanshinone IIA in the treatment of astrocytoma. This study aimed to investigate the effect of tanshinone IIA on migration, proliferation and apoptosis of astrocytoma cells. The efficacy of tanshinone IIA on migration, proliferation and apoptosis of astrocytoma cells were evaluated by flow cytometry and the assays of plate clone formation, CCK-8, wound healing and transwell migration. The protein molecule and signaling pathway were detected by western blot. High-dose tanshinone IIA suppressed migration and proliferation of astrocytoma cells while promoting apoptosis of astrocytoma cells. The western blot results showed that there were high Notch-1 protein expression and low c-Myc, MMP-9 and Bcl-2 activation in the high-dose tanshinone IIA group compared with the control group. High-dose tanshinone IIA suppresses astrocytoma cell proliferation, migration while promoting apoptosis through Notch-1 pathway. Tanshinone IIA may be used to develop new drugs for the treatment of astrocytoma.
KeywordsTanshinone IIA Astrocytoma Notch-1 pathway MMP-9 C-Myc, Bcl-2
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- 12.Li F, Han G, Wu K (2016) Tanshinone IIA alleviates the AD phenotypes in APP and PS1 transgenic mice. BioMed Res Int 2016:8Google Scholar
- 28.Sharma I, Singh A, Sharma KC, Saxena S (2017) Gene expression profiling of chemokines and their receptors in low and high grade astrocytoma. Asian Pac J Cancer Prevent 18(5):1307–1313Google Scholar
- 31.Fan G, Zhu Y, Guo H, Wang X, Wang H, Gao X (2011) Direct vasorelaxation by a novel phytoestrogen tanshinone IIA is mediated by nongenomic action of estrogen receptor through endothelial nitric oxide synthase activation and calcium mobilization. J Cardiovasc Pharmacol 57:340–347CrossRefPubMedGoogle Scholar