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Neurochemical Research

, Volume 43, Issue 9, pp 1855–1861 | Cite as

High-Dose Tanshinone IIA Suppresses Migration and Proliferation While Promoting Apoptosis of Astrocytoma Cells Via Notch-1 Pathway

  • Wanliang Dong
  • Yuankun Zhang
  • Xuemei Chen
  • Yanjie Jia
Original Paper
  • 34 Downloads

Abstract

Malignant astrocytoma is the most common malignant tumor with strong invasion in the central nervous system. Tanshinone IIA is an effective compound to suppress cell proliferation and promote cell apoptosis. However, there is little research about the role of tanshinone IIA in the treatment of astrocytoma. This study aimed to investigate the effect of tanshinone IIA on migration, proliferation and apoptosis of astrocytoma cells. The efficacy of tanshinone IIA on migration, proliferation and apoptosis of astrocytoma cells were evaluated by flow cytometry and the assays of plate clone formation, CCK-8, wound healing and transwell migration. The protein molecule and signaling pathway were detected by western blot. High-dose tanshinone IIA suppressed migration and proliferation of astrocytoma cells while promoting apoptosis of astrocytoma cells. The western blot results showed that there were high Notch-1 protein expression and low c-Myc, MMP-9 and Bcl-2 activation in the high-dose tanshinone IIA group compared with the control group. High-dose tanshinone IIA suppresses astrocytoma cell proliferation, migration while promoting apoptosis through Notch-1 pathway. Tanshinone IIA may be used to develop new drugs for the treatment of astrocytoma.

Keywords

Tanshinone IIA Astrocytoma Notch-1 pathway MMP-9 C-Myc, Bcl-2 

Notes

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Wanliang Dong
    • 1
  • Yuankun Zhang
    • 2
  • Xuemei Chen
    • 3
  • Yanjie Jia
    • 1
  1. 1.Neurology DepartmentThe First Affiliated Hospital of Zhengzhou UniversityZhengzhouChina
  2. 2.Vasculocardiology DeparmentZhengzhou People’s HospitalZhengzhouChina
  3. 3.Department of Anatomy of the School of Basic Medical SciencesZhengzhou UniversityZhengzhouChina

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