Localization of Mitochondrial Carnitine/Acylcarnitine Translocase in Sensory Neurons from Rat Dorsal Root Ganglia
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The carnitine/acylcarnitine transporter is a transport system whose function is essential for the mitochondrial β-oxidation of fatty acids. Here, the presence of carnitine/acylcarnitine carrier (CACT) in nervous tissue and its sub-cellular localization in dorsal root ganglia (DRG) neurons have been investigated. Western blot analysis using a polyclonal anti-CACT antibody produced in our laboratory revealed the presence of CACT in all the nervous tissue extracts analyzed. Confocal microscopy experiments performed on fixed and permeabilized DRG neurons co-stained with the anti-CACT antibody and the mitochondrial marker MitoTracker Red clearly showed a mitochondrial localization for the carnitine/acylcarnitine transporter. The transport activity of CACT from DRG extracts reconstituted into liposomes was about 50 % in respect to liver extracts. The experimental data here reported represent the first direct evidence of the expression of the carnitine/acylcarnitine transporter in sensory neurons, thus supporting the existence of the β-oxidation pathway in these cells.
KeywordsSensory neurons DRG Mitochondria Carnitine/acylcarnitine transporter Immunohistochemistry β-Oxidation
This work was partially supported by founds from Programma Operativo Nazionale 01_00937-MIUR.
Conflict of interest
The authors declare that they have no conflict of interest.
Supplementary movie 1 Three-dimensional reconstruction of the mitochondrial network close to the cell body of the same sensory neuron depicted in Supplementary Figure 1 (ROI). Z-stack confocal images were 3D-projected with the Fiji plugin “3D viewer”. Video shows 360° view of image reconstruction. (MPG 3,188 kb)
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