Neuroscience and Behavioral Physiology

, Volume 49, Issue 9, pp 1100–1105 | Cite as

An Immunohistochemical Study of the Pathways of the Influence of Dopamine on Orexinergic Neurons in the Perifornical Area of the Hypothalamus

  • I. Yu. Morina
  • A. L. Mikhrina
  • I. V. RomanovaEmail author

Confocal microscopy was used to analyze double immunolabeling and provided evidence of the locations of a large number of tyrosine hydroxylase-immunopositive processes around the bodies of orexinergic neurons located in the perifornical area of the hypothalamus in rats. The bodies of orexinergic neurons were found to bear dopamine D1 receptors. A high level of colocalization of dopamine D1 and D2 receptors was found in the perifornical area, which is evidence for the formation of heterodimeric D1/D2 complexes. After i.p. administration of the selective D1 receptor antagonist SCH 39166, neurons in the perifornical area showed increases in the optical density of c-Fos protein in both orexinergic neurons and their adjacent GABA neurons. These data provide evidence that dopamine is able to influence orexinergic neurons both via a direct route involving D1- and D2-dependent signal pathways and via influences on GABA neurons.


brain orexins hypothalamus dopamine dopamine D1 and D2 receptors GABA 


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • I. Yu. Morina
    • 1
  • A. L. Mikhrina
    • 1
  • I. V. Romanova
    • 1
    Email author
  1. 1.Sechenov Institute of Evolutionary Physiology and BiochemistryRussian Academy of SciencesSt. PetersburgRussia

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