Use of Hydrobionts as Alternative Biological Models

  • G. I. ProninaEmail author
  • N. Yu. Koryagina
  • A. O. Revyakin
  • O. I. Stepanova
  • Zh. O. Kurishenko
  • N. V. Petrova

We present here results from model experiments with poikilotherm hydrobionts belonging to different taxonomic groups: crayfish (Pontatacus leptodactylus), fish, i.e., carp (Cyprinus carpio), and amphibians, i.e., axolotl (Ambystoma mexicanum). Fatty dystrophy of the hepatopancreas in crayfish and pathology of the pancreas in carp were modeled by administration of alloxan at doses of 50 and 200 mg/kg, respectively. Crayfish received alloxan into the ventral sinus and fish were treated i.p. Liver pathology in fish was modeled by administration of seven doses of paracetamol 15 g/kg over 14 days. Parenteral administration (into the central sinus of crayfish, i.v. in fish, and i.p. in axolotls) of stem and progenitor cells from mammalian donors (mice) at a dose of 10·106 bone marrow cells (BMC) was found to have effects. Fish and crayfish with pathology displayed intensive tissue regeneration and restoration of damaged parenchymatous organs. Administration of stem cells accelerated the process of regeneration of amputated limbs in axolotls. These results extend the potential of medical-biological studies of in vivo models.


alternative biological models hydrobionts fish crayfish axolotls hepatopancreas stem cells 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • G. I. Pronina
    • 1
    Email author
  • N. Yu. Koryagina
    • 1
  • A. O. Revyakin
    • 2
  • O. I. Stepanova
    • 2
  • Zh. O. Kurishenko
    • 2
  • N. V. Petrova
    • 2
  1. 1.All-Russian Research Institute of Irrigation Fish FarmingVorovskii SettlementMoscow RegionRussia
  2. 2.Scientific Center for Biomedical TechnologiesFederal Medical Biological AgencyMoscow RegionRussia

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