Slow-Wave Sleep and Anxiety Levels in Rats in Chronic Deficiency of Chaperone Hsp70i in the Preoptic Area of the Hypothalamus
- 1 Downloads
The preoptic area of the hypothalamus (POA) is regarded as an integrative “center” regulating sleep and behavior, though the involvement of chaperones in the molecular mechanisms controlling the sleep–waking cycle and anxiety-like behavior has received little study. Using a model based on suppression of the synthesis of inducible chaperone Hsp70i in the POA of rats using RNA interference, the present studies provided the first demonstration that long-term (1–2.5 months) 45–55% decreases in Hsp70i levels in the POA leads to reductions in slow-wave sleep in the dark phase of the day and increases in the level of anxiety. The study results may be of clinical value in seeking means of mobilizing the activity of chaperones for the treatment of sleep and behavioral disorders, particularly in aging and a number of neuropathologies, which are characterized by an imbalance in the chaperone systems and decreases in slow-wave sleep.
Keywordspreoptic area of the hypothalamus chaperone Hsp70 slow-wave sleep anxiety-like behavior RNA interference rats
Unable to display preview. Download preview PDF.
- 1.E. V. Verbitskii, “Anxiety and sleep,” Zh. Vyssh. Nerv. Deyat., 63, No. 1, 6–12 (2013).Google Scholar
- 2.I. V. Ekimova, “The somnogenic effect of 70 kDa exogenous heat shock protein is mediated via GABAA receptors in the preoptic area of the hypothalamus,” Dokl. Akad. Nauk., 449, No. 6, 725 (2013).Google Scholar
- 3.Yu. F. Pastukhov, “Slow-wave sleep and molecular chaperones,” Zh. Evolyuts. Biokhim. Fiziol., 52, No. 1, 79–90 (2016).Google Scholar
- 4.Yu. F. Pastukhov, I. V. Ekimova, D. A. Meshalkina, et al., “Studies of the involvement of Hdj1 cochaperone in modulating sleep and behavior using in vivo microRNA techniques,” Ros. Fiziol. Zh., 98, No. 12, 1530–1543 (2012).Google Scholar
- 5.Yu. F. Pastukhov, I. V. Ekimova, K. A. Khudik, and I. V. Guzhova, “70 kDa protein in the regulation of sleep and thermal regulation,” Zh. Evolyuts. Biokhim. Fiziol., 44, No. 1, 65–71 (2008).Google Scholar
- 6.Yu. F. Pastukhov, V. V. Simonova, M. A. Guzeev, et al., “Chaperone Hsp70 is involved in the molecular mechanisms regulating slow-wave sleep,” Dokl. Akad. Nauk., 461, No. 2, 228–231 (2015).Google Scholar
- 7.Yu. F. Pastukhov, M. V. Chernyshev, I. V. Ekimova, and K. A. Khudik, “Molecular chaperones – new modulators of anxiety and sleep homeostasis,” in: Sleep and Anxiety, E. V. Verbitskii (ed.), Southern Science Center, Russian Academy of Sciences, Rostov-on-Don (2008), pp. 235–268.Google Scholar