Neuroscience and Behavioral Physiology

, Volume 49, Issue 3, pp 379–383 | Cite as

Developmental Changes in NO-Containing Sympathetic Neurons in the Spinal Cord in Rats

  • K. Yu. Moiseyev
  • P. M. MasliukovEmail author

Objectives. To determine the location, percentage composition, and morphometric properties of sympathetic preganglionic neurons containing NO synthase (NOS) in the spinal cord in rats. Materials and methods. Studies were performed on 35 white female Wistar rats aged 3, 10, 20, 30, and 60 days, six months, and three years. Spinal cord sections made at the level of segment T2 were used for immunohistochemical detection of NOS and the acetylcholine-synthesizing enzyme choline acetyltransferase (CAT). The areas of nerve cells and the proportions of immunoreactive neurons were determined. Results. Most sympathetic preganglionic neurons in the spinal cords of neonatal and 10-day-old rats contained NOS and also CAT. Rats of these age groups also contained a population of NOS-positive/CAT-negative neurons (26% in neonates and 8% in 10-day-old animals), which was not seen in older animals. During the first 20 days, the proportion of NOS-immunopositive neurons decreased significantly, while that of CAT-positive neurons increased. Conclusions. In early postnatal ontogeny there was a decrease in the number of sympathetic preganglionic neurons expressing NOS, which may have influences on the mechanisms of NO-ergic sympathetic transmission.


sympathetic preganglionic neurons NO synthase spinal cord immunohistochemistry rats 


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  1. 1.
    E. A. Kolos and D. E. Korzhevskii, “Distribution of cholinergic and nitroxidergic neurons in the spinal cord of neonatal and adult rats,” Morfologiya, 147, No. 2, 32–37 (2015).Google Scholar
  2. 2.
    P. M. Masliukov, A. I. Emanuilov, and A. D. Nozdrachev, “Developmental changes in the neurotransmitter composition of neurons in the sympathetic ganglia,” Usp. Gerontol., 29, No. 3, 442–453 (2016).Google Scholar
  3. 3.
    G. F. Sitdikova, A. V. Yakovlev, and A. L. Zefi rov, “Gaseous transmitters: from toxic effects to the regulation cell functions and clinical exploitation,” Byull. Sibirsk. Med., 13, No. 6, 185–200 (2014).Google Scholar
  4. 4.
    M. Cossenza, R. Socodato, C. C. Portugal, et al., “Nitric oxide in the nervous system: biochemical, developmental, and neurobiological aspects,” Vitam. Horm., 96, 79–125 (2014).CrossRefGoogle Scholar
  5. 5.
    S. A. Deuchars and V. K. Lall, “Sympathetic preganglionic neurons: properties and inputs,” Compr. Physiol., 5, No. 2, 829–869 (2015).CrossRefGoogle Scholar
  6. 6.
    A. I. Emanuilov, M. B. Korzina, L. I. Archakova, et al., “Development of the NADPH-diaphorase-positive neurons in the sympathetic ganglia,” Ann. Anat., 190, No. 6, 516–524 (2008).CrossRefGoogle Scholar
  7. 7.
    J. P. Foong, “Postnatal development of the mouse enteric nervous system,” Adv. Exp. Med. Biol., 891, 135–143 (2016).CrossRefGoogle Scholar
  8. 8.
    J. Garthwaite, “From synaptically localized to volume transmission by nitric oxide,” J. Physiol., 594, 9–18 (2016).CrossRefGoogle Scholar
  9. 9.
    P. M. Masliukov, A. I. Emanuilov, L. V. Madalieva, et al., “Development of nNOS-positive neurons in the rat sensory and sympathetic ganglia,” Neuroscience, 256, 271–281 (2014).CrossRefGoogle Scholar
  10. 10.
    P. M. Masliukov, M. M. Fateev, and A. D. Nozdrachev, “Age-dependent changes of electrophysiologic characteristics of the stellate ganglion conducting pathways in kittens,” Auton. Neurosci., 83, No. 1–2, 12–18 (2000).CrossRefGoogle Scholar
  11. 11.
    P. E. Phelps, R. P. Barber, and J. E. Vaughn, “Embryonic development of choline acetyltransferase in thoracic spinal motor neurons: somatic and autonomic neurons may be derived from a common cellular group,” J. Comp. Neurol., 307, No. 1, 77–86 (1991).CrossRefGoogle Scholar
  12. 12.
    A. Philippu, “Nitric oxide: a universal modulator of brain function,” Curr. Med. Chem., 23, 2643–2652 (2016).CrossRefGoogle Scholar
  13. 13.
    J. P. Timmermans, M. Barbiers, D. W. Scheuermann, et al., “Distribution pattern, neurochemical features and projections of nitrergic neurons in the pig small intestine,” Ann. Anat., 176, 515–525 (1994).CrossRefGoogle Scholar
  14. 14.
    R. Wetts and J. E. Vaughn, “Choline acetyltransferase and NADPH diaphorase are co-expressed in rat spinal cord neurons,” 63, No. 4, 1117–1124 (1994).Google Scholar

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Normal Physiology with Biophysics, Yaroslavl State Medical UniversityRussian Federation Ministry of HealthYaroslavlRussia

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