Biocompatibility of nitrogen-doped multiwalled carbon nanotubes with murine fibroblasts and human hematopoietic stem cells

  • Jose G. Munguia-Lopez
  • Rodrigo Juarez
  • Emilio Muñoz-Sandoval
  • Marco A. Kalixto-Sanchez
  • Joseph Matthew Kinsella
  • Antonio De Leon-RodriguezEmail author
Research Paper


Chemical vapor deposition (CVD) methods to create carbon nanotubes (CNTs) with specific dopant atoms have been of interest in biomedical applications due to the relative ease of synthesis of doped CNTs with controlled physical properties. However, CNTs generated from CVD are often heterogeneous in chemical functionality, size, aspect ratio, number of walls, and conducting properties resulting in potential inconsistencies during measurement of the physiological activity of cell-CNT interactions. In this work, the biocompatibility of nitrogen-doped multiwalled carbon nanotubes (CNx) with both murine fibroblasts and human hematopoietic stem cells (hHSC) was evaluated. CNx were synthesized by CVD, purified, characterized, and classified into three fractions designated as small-CNx (S-CNx), medium (M-CNx), and large (L-CNx). Mammalian cells were incubated with CNx doses between 0.07 and 70 μg/mL, and cell viability was evaluated. hHSC and murine fibroblast both demonstrated non-significant differences in proliferation rates when exposed to M-CN, whereas, either cells experienced inhibited growth following exposure to either S-CNx and L-CNx under the same conditions. In this work, it has been demonstrated that CNTs produced by CVD have differences on the biocompatibility with mammalian cells, but the M-CNx could be a great candidate for biomedical applications.


Nanomaterials Nitrogen-doped carbon nanotubes Murine cells Stem cells Biomedical applications 



The authors thank L. Aldana and Abdullah Chaudhary for the English review and V. Balderas for his technical assistance.


This research was funded by The Marcos Moshinsky Foundation and CONACYT-Mexico grant number CB-2013-220744 and 250279.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11051_2019_4637_MOESM1_ESM.docx (294 kb)
ESM 1 (DOCX 293 kb)


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Molecular BiologyInstituto Potosino de Investigación Científica y Tecnológica, A.C. San Luis PotosíMexico
  2. 2.Department of BioengineeringMcGill UniversityMontrealCanada
  3. 3.Advanced Materials DepartmentInstituto Potosino de Investigación Científica y Tecnológica, A.C. San Luis PotosíMexico
  4. 4.Hospital General del ISSSTESan Luis PotosiMexico

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