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Toxicity and biodegradation of zinc ferrite nanoparticles in Xenopus laevis

  • M. Rivero
  • M. Marín-Barba
  • L. Gutiérrez
  • E. Lozano-Velasco
  • G. N. Wheeler
  • J. Sánchez-Marcos
  • A. Muñoz-Bonilla
  • C. J. Morris
  • A. RuizEmail author
Research Paper

Abstract

Zn-doped Fe3O4 magnetic nanoparticles have been proposed as the ideal ferrite for some biomedical applications like magnetic hyperthermia or photothermal therapy because of the possibility to adjust their size and chemical composition in order to design tailored treatments. However, reliable approaches are needed to risk assess Zn ferrite nanoparticles before clinical development. In this work, the in vitro toxicity of the nanoparticles was evaluated in five cellular models (Caco-2, HepG2, MDCK, Calu-3 and Raw 264.7) representing different target organs/systems (gastrointestinal system, liver, kidney, respiratory system and immune system). For the first time, these nanoparticles were evaluated in an in vivo Xenopus laevis model to study whole organism toxicity and their impact on iron and zinc metabolic pathways. Short- and long-term in vivo exposure studies provided insights into the contrasting adverse effects between acute and chronic exposure. Quantitative PCR combined with elemental analysis and AC magnetic susceptibility measurements revealed that at short-term exposure (72 h), the nanoparticles’ absorption process is predominant, with the consequent over-expression of metal transporters and metal response proteins. At long-term exposure (120 h), there is an upregulation of metal accumulation involved genes and the return to basal levels of both iron and zinc transporters, involved in the uptake of metals. This suggests that at this stage, the nanoparticles’ absorption process is residual compared with the following steps in metabolism, distribution and/or excretion processes, indicated by the increase of iron accumulation proteins at both transcriptional and translational level. This testing approach based on a battery of cellular systems and the use of the Xenopus laevis model could be a viable strategy for studying the toxicity, degradability and ultimately the long-term fate of zinc ferrites in the organism.

Graphical abstract

Biodegradation of Zn ferrite NPs have been studied. Quantitative PCR combined with elemental analysis and AC magnetic susceptibility measurements revealed that at short-term exposure (72 h), the nanoparticles’ absorption process is predominant, with the consequent over-expression of metal transporters and metal response proteins. At long-term exposure (120 h), there is an upregulation of metal accumulation involved genes and the return to basal levels of both iron and zinc elements in the body. This suggests that at this stage, the nanoparticles’ absorption process is residual compared with the following steps in metabolism, distribution and/or excretion processes, indicated by the increase of iron accumulation proteins at both transcriptional and translational level.

Keywords

Zinc ferrite nanoparticles Xenopus laevis Toxicity Biodegradation Metabolism Environmental and health effects 

Notes

Acknowledgments

Servicio General de Apoyo a la Investigación-SAI, Universidad de Zaragoza is acknowledged. The authors also acknowledge the facilities and the scientific and technical assistance, especially that of Bertrand Leze from the SEM service of the University of East Anglia.

Funding information

M. Marín-Barba has been supported by the People Program (Marie Curie Actions) of the European Union’s Seventh Framework Program FP7 under REA grant agreement number 607142 (DevCom). A. Ruiz was supported by FP7-People Framework – Marie Curie Industry and Academia Partnerships & Pathways scheme (DNA-TRAP project, grant agreement no. 612338). L. Gutiérrez recognizes financial support from the Ramón y Cajal subprogram (RYC-2014-15512). E. Lozano-Velasco acknowledges support from Marie Curie fellowship (705089-MIR-CHROM-C).

Compliance with ethical standards

All experiments were performed in compliance with the relevant laws and institutional guidelines at the University of East Anglia. The research has been approved by the local ethical review committee according to UK Home Office regulations.

Conflict of interest

The authors declare that there are no conflicts of interest.

Supplementary material

11051_2019_4631_MOESM1_ESM.docx (686 kb)
ESM 1 (DOCX 686 kb)

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© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Synthesis and Electrochemical and Spectroscopic Characterization of Materials Group, Departamento de Química-Física Aplicada, Facultad de CienciasUniversidad Autónoma de MadridMadridSpain
  2. 2.School of Biological SciencesUniversity of East AngliaNorwichUK
  3. 3.Departmento de Química Analítica, Instituto de Ciencia de Materiales de Aragón (ICMA/CSIC)Universidad de Zaragoza and CIBER-BBNZaragozaSpain
  4. 4.Macromolecular Engineering Group, Instituto de Ciencia y Tecnología de Polímeros (ICTP-CSIC)MadridSpain
  5. 5.School of PharmacyUniversity of East AngliaNorwichUK
  6. 6.School of PharmacyQueen’s University BelfastBelfastUK

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