Synthesis, characterization, and toxicity of hollow gold nanoshells
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Hollow gold nanoshells (HGN) with a diameter of 50–70 nm and tunable optical properties within the near-infrared region were synthesized from a substitution reaction using a sacrificial template, in which the morphological properties of the HGN were affected by the synthesis conditions. Using EDX line scan, the composition of the structure was determined to verify if the sacrificial template is completely consumed or residues remain after the chemical synthesis, obtaining that the final HGN structure contains about 11% of the remaining silver that showed no significant effect on the cell viability of a hNS1 cell line, but resulted as toxic on a C6 glioma cell line at high concentrations. The photothermal properties were evaluated using a NIR laser, which despite its low power showed the conversion of light into heat. This study was conducted to evaluate the potential of these nanostructures as therapeutic agents with an emphasis on toxicity.
KeywordsPhotothermal therapy Hollow gold nanoshells Surface plasmon resonance EDX line scan Toxicity Silver residues
This work was funded by “Fondo de Apoyo a la Investigacion, UASLP” (C14-FAI-04-23.23) and by “Fondo Sectorial de Investigacion para la Educacion, CONACYT” (CB-258444-2016).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Han C, Yang J, Gu J (2018) Immobilization of silver nanoparticles in Zr-based MOFs: induction of apoptosis in cancer cells. J Nanopart Res 20. https://doi.org/10.1007/s11051-018-4187-5
- Kumar R, Maitra AN, Patanjali PK, Sharma P (2005) Hollow gold nanoparticles encapsulating horseradish peroxidase. Biomaterials 26:6743–6753. https://doi.org/10.1016/j.biomaterials.2005.04.045 CrossRefGoogle Scholar
- Lindley SA, Cooper JK, Rojas-Andrade MD, Fung V, Leahy CJ, Chen S, Zhang JZ (2017) Highly tunable hollow gold nanospheres: gaining size control and uniform galvanic exchange of sacrificial cobalt boride scaffolds. ACS Appl Mater Interfaces 10:12992–13001. https://doi.org/10.1021/acsami.8b00726 CrossRefGoogle Scholar
- Lu W, Huang Q, Ku G, Wen X, Zhou M, Guzatov D, Brecht P, Su R, Oraevsky A, Wang LV, Li C (2010) Photoacoustic imaging of living mouse brain vasculature using hollow gold nanospheres. Biomaterials 31:2617–2626. https://doi.org/10.1016/j.biomaterials.2009.12.007 CrossRefGoogle Scholar
- Lux F, Lerouge F, Bosson J, Lemercier G, Andraud C, Vitrant G, Baldeck PL, Chassagneux F, Parola S (2009) Gold hollow spheres obtained using an innovative emulsion process: towards multifunctional Au nanoshells. Nanotechnology 20:355603. https://doi.org/10.1088/0957-4484/20/35/355603 CrossRefGoogle Scholar
- Melancon MP, Lu W, Yang Z, Zhang R, Cheng Z, Elliot AM, Stafford J, Olson T, Zhang JZ, Li C (2008) In vitro and in vivo targeting of hollow gold nanoshells directed at epidermal growth factor receptor for photothermal ablation therapy. Mol Cancer Ther 7:1730–1739. https://doi.org/10.1158/1535-7163.MCT-08-0016 CrossRefGoogle Scholar
- Pfaller T, Colognato R, Nelissen I, Favilli F, Casals E, Ooms D, Leppens H, Ponti J, Stritzinger R, Puntes V, Boraschi D, Duschl A, Oostingh GJ (2010) The suitability of different cellular in vitro immunotoxicity and genotoxicity methods for the analysis of nanoparticle-induced events. Nanotoxicology 4:52–72. https://doi.org/10.3109/17435390903374001 CrossRefGoogle Scholar
- Poudel BK, Gupta B, Ramasamy T, Thapa RK, Pathak S, Oh KT, Jeong JH, Choi HG, Yong CS, Kim JO (2017) PEGylated thermosensitive lipid-coated hollow gold nanoshells for effective combinational chemo-photothermal therapy of pancreatic cancer. Colloids Surf B 160:73–83. https://doi.org/10.1016/j.colsurfb.2017.09.010 CrossRefGoogle Scholar
- Preciado-Flores S, Wang D, Wheeler DA, Newhouse R, Hensel JK, Schwartzberg A, Wang L, Zhu J, Barboza-Flores M, Zhang JZ (2011) Highly reproducible synthesis of hollow gold nanospheres with near infrared surface plasmon absorption using PVP as stabilizing agent. J Mater Chem 21:2344–2350. https://doi.org/10.1039/c0jm03690k CrossRefGoogle Scholar
- Yu M, Guo F, Wang J, Tan F, Li N (2016) A pH-driven and photoresponsive nanocarrier: remotely-controlled by near-infrared light for stepwise antitumor treatment. Biomaterials 79:25–35. https://doi.org/10.1016/j.biomaterials.2015.11.049 CrossRefGoogle Scholar