Development and characterization of PLGA nanoparticles containing antibiotics
- 103 Downloads
Several strategies for the delivery and release of drugs have been studied, among them the use of polymeric nanoparticles of PLGA (poly lactic-co-glycolic acid). These nanoparticles (NPs) have been shown to be promising for the controlled and effective release of drugs due to their biodegradability and biocompatibility. Regarding this, the aim of this study is to synthesize and characterize PLGA polymer nanoparticles associated with antimicrobials in order to reduce adverse reactions and to have a more effective local delivery. Empty PLGA nanoparticles and conjugated to vancomycin and meropenem antibiotics were synthesized by the double emulsification-solvent evaporation technique. Then, they were characterized by the analysis of the mean particle diameter, dynamic light scattering (DLS), Fourier transform infrared (FTIR) vibrational spectroscopy, contact angle measurement, and atomic force microscopy (AFM) and scanning electron microscopy (SEM). The DLS analysis of the NPs obtained showed approximate sizes of 263.5 nm (NPs-PLGA), 239.3 nm (NPs-VAN) and 284.2 nm (NPs-MER), and monodispersivity. FTIR results and contact angle measurements suggest encapsulation of antibiotics to NPs. The morphology evaluated through AFM and SEM indicates homogeneous, uniform, and spherical distribution of NPs and also apparently smooth. The antibacterial action of PLGA nanoparticles carried with antibiotics was effective when using concentrations of 5–2.5 mg/mL (PLGA-VAN) versus Staphylococcus aureus and 10–2.5 mg/mL (PLGA-MER) to Pseudomonas aeruginosa. The release kinetics of the antibiotics revealed a release profile of 43.9% at the end of 24 h and 96% at the end of 96 h for PLGA-VAN formulation and 8.25% and 16% at the end of 24 h and 96 h, for PLGA-MER, respectively. This study supports the potential application of PLGA particles containing antibiotics as facilitators of drug delivery and release effectively.
KeywordsNanoparticles PLGA Antibiotics Drug delivery application
Compliance with ethical standards
Conflict of interest
The authors have also confirmed that this article is unique and not under consideration or published in any other publication, and that they have permission from rights holders to reproduce any copyrighted material. Any disclosures are made in this section. The external blind peer reviewers report no conflicts of interest.
- Clinical and Laboratory Standards Institute (2017) M100-S27. Performance standards for antimicrobial susceptibility testing: 27th informational supplement. CLSI, WayneGoogle Scholar
- Georgiev AG, Johansen J, Ramanathan VD, Sere YY, Beh CT, Menon AK (2013) Arv1 regulates PM and ER membrane structure and homeostasis but is dispensable for intracellular sterol transport. Traffic 14(8):912–921Google Scholar
- Rao JP, Geckeler KE (2011) Polymer nanoparticles: preparation techniques and size-control parameters. Prog Polym Sci 36:887–913. https://doi.org/10.1016/j.progpolymsci.2011.01.001 CrossRefGoogle Scholar
- Silva ISM, Santos RFP, Melo TVC, Silva AJC, Sarmento PA, Lúcio IML, Campesatto EA, Padilha FF, Conserva LM, Bastos MLA (2014) In vitro biological potential of Guanxuma-of-horn [Sebastiania corniculata (Vahl) Mull. Arg.] in infection control. J Chem Pharm Res 6(4):663–669Google Scholar
- Tomaszewska E, Soliwoda K, Kadziola K, Tkacz-Szczesna B, Celichowski G, Cichomski M, Szmaja W, Grobelny J (2013) Detection limits of DLS and UV-vis spectroscopy in characterization of Polydisperse nanoparticles colloids. J Nanomater 2013:1–10. https://doi.org/10.1155/2013/313081 CrossRefGoogle Scholar
- Venkatesh DN, Baskaran M, Karri VVSR, Mannemala SS, Radhakrishna K, Goti S (2015) Fabrication and in vivo evaluation of Nelfinavir loaded PLGA nanoparticles for enhancing oral bioavailability and therapeutic effect. Saudi Pharm J 23(6):667–674. https://doi.org/10.1016/j.jsps.2015.02.021 CrossRefGoogle Scholar
- World Health Organization (WHO) (2017) Global priority list of antibiotic-resistant bacteria to guide research, discovery, and development of new antibiotics. IOP Publishing PhysicsWeb. http://www.who.int/medicines/publications/global-priority-list-antibiotic-resistant-bacteria/en/. Accessed 10 December 2017
- Zakeri-Milani P, Loveymi BD, Jelvehgari M, Valizadeh H (2013) The characteristics and improved intestinal permeability of vancomycin PLGA-nanoparticles as colloidal drug delivery system. Colloids Surf B: Biointerfaces 103:174–181. https://doi.org/10.1016/j.colsurfb.2012.10.021 CrossRefGoogle Scholar