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Thermally sensitive polypeptide-based copolymer for DNA complexation into stable nanosized polyplexes

  • Emilya Ivanova
  • Ivaylo DimitrovEmail author
  • Rahila Kozarova
  • Sevdalina Turmanova
  • Margarita Apostolova
Research Paper

Abstract

Gene therapy based on non-viral synthetic delivery vectors has attracted much attention in the past two decades. However, it is still in clinical trial stages, mainly due to the lack of safe and efficient delivery vehicles. Herein, we report on the synthesis and DNA complexation ability of novel, hybrid copolymer comprising poly(N-isopropylacrylamide) (PNIPAm) block with poly(ethylene glycol) (PEG) side chains and a polycationic block of poly(l-lysine) (PLLys). The copolymer was synthesized in a two-step procedure. In the first step, a thermally sensitive PNIPAm-g-PEG copolymer with terminal ammonium hydrochloride group was prepared. The second step involves controlled ring-opening polymerization of Z-l-lysine N-carboxyanhydride initiated by the PNIPAm-g-PEG macroinitiator. The hybrid copolymer obtained show high ability to condense DNA into stable polyplexes with sizes below 100 nm. Cytotoxicity evaluation of both hybrid copolymer and its polyplex with DNA indicates that it might be a good candidate for gene-delivery applications.

Keywords

DNA Polyplexes Stimuli-sensitive polymers Synthesis 

Notes

Acknowledgments

This study was financially supported by the Bulgarian National Science Fund through a project “Ideas” DO 02-247/2008.

Supplementary material

11051_2012_1358_MOESM1_ESM.doc (98 kb)
Electronic supplementary material (DOC 98 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Emilya Ivanova
    • 1
    • 3
  • Ivaylo Dimitrov
    • 1
    Email author
  • Rahila Kozarova
    • 2
  • Sevdalina Turmanova
    • 3
  • Margarita Apostolova
    • 2
  1. 1.Institute of Polymers, Bulgarian Academy of SciencesSofiaBulgaria
  2. 2.Institute of Molecular Biology, Bulgarian Academy of SciencesSofiaBulgaria
  3. 3.Department of Materials ScienceProf. Assen Zlatarov UniversityBurgasBulgaria

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