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Demonstration of Adventitious Sporulation in Fusarium Petroliphilum Onychomycosis

  • Ziauddin KhanEmail author
  • Suhail Ahmad
  • Wadha Alfouzan
  • Leena Joseph
  • Soumya Varghese
Original Paper
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Abstract

Fusarium petroliphilum is a recently described species within the Fusarium solani species complex. Some strains of Fusarium species are capable of forming yeast-like structures in tissue as well as in culture through a process known as “adventitious sporulation.” Here, we describe the formation of these yeast-like reproductive structures in infected nail tissue obtained from a case of onychomycosis. These structures were also observed in culture grown on RPMI 1640 agar supplemented with 2% glucose. The isolate was resistant to azoles and echinocandins. To the best of our knowledge, this is the first report describing adventitious sporulation in F. petroliphilum and its etiologic role in onychomycosis.

Keywords

Fusarium petroliphilum Adventitious sporulation Yeast-like structures Antifungal resistance 

Notes

Acknowledgements

Technical support received from Omar Al-Musallam is thankfully acknowledged.

Compliance with Ethical Standards

Conflict of interest

Authors report no conflict interest.

References

  1. 1.
    Al-Hatmi AM, Meis JF, de Hoog GS. Fusarium: molecular diversity and intrinsic drug resistance. PLoS Pathog. 2016;12:e1005464.CrossRefGoogle Scholar
  2. 2.
    Al-Hatmi AM, Hagen F, Menken SB, Meis JF, de Hoog GS. Global molecular epidemiology and genetic diversity of Fusarium, a significant emerging group of human opportunists from 1958 to 2015. Emerg Microbes Infect. 2016;5(12):e124.PubMedPubMedCentralGoogle Scholar
  3. 3.
    Guarro J. Fusariosis, a complex infection caused by a high diversity of fungal species refractory to treatment. Eur J Clin Microbiol Infect Dis. 2013;32:1491–500.CrossRefGoogle Scholar
  4. 4.
    O’Donnell K, Sutton DA, Fothergill A, McCarthy D, Rinaldi MG, Brandt ME, Zhang N, Geiser DM. Molecular phylogenetic diversity, multilocus haplotype nomenclature, and in vitro antifungal resistance within the Fusarium solani species complex. J Clin Microbiol. 2008;46:2477–90.CrossRefGoogle Scholar
  5. 5.
    Salah H, Al-Hatmi AM, Theelen B, Abukamar M, Hashim S, van Diepeningen AD, Lass-Florl C, Boekhout T, Almaslamani M, Taj-Aldeen SJ. Phylogenetic diversity of human pathogenic Fusarium and emergence of uncommon virulent species. J Infect. 2015;71:658–66.CrossRefGoogle Scholar
  6. 6.
    Short DP, O’Donnell K, Thrane U, Nielsen KF, Zhang N, Juba JH, Geiser DM. Phylogenetic relationships among members of the Fusarium solani species complex in human infections and the descriptions of F. keratoplasticum sp. nov. and F. petroliphilum stat nov. Fungal Genet Biol. 2013;53:59–70.CrossRefGoogle Scholar
  7. 7.
    Chen QT, Fu XH. Two petroliphilous taxa of Fusarium. Acta Mycol Sin. 1987;Suppl. 1:328–33.Google Scholar
  8. 8.
    Ersal T, Al-Hatmi ASM, Cilo BD, et al. Fatal disseminated infection with Fusarium petroliphilum. Mycopathologia. 2015;179:119–24.CrossRefGoogle Scholar
  9. 9.
    Guevara-Suarez M, Cano-Lira JF, de Garcia MC, Sopo L, De Bedout C, Cano LE, García AM, Motta A, Amézquita A, Cárdenas M, Espinel-Ingroff A, Guarro J, Restrepo S, Celis A. Genotyping of Fusarium isolates from onychomycoses in Colombia: detection of two new species within the Fusarium solani species complex and in vitro antifungal susceptibility testing. Mycopathologia. 2016;181:165–74.CrossRefGoogle Scholar
  10. 10.
    Moretti ML, Busso-Lopes AF, Tararam CA, Moraes R, Muraosa Y, Mikami Y, Gonoi T, Taguchi H, Lyra L, Reichert-Lima F, Trabasso P, de Hoog GS, Al-Hatmi AMS, Schreiber AZ, Kamei K. Airborne transmission of invasive fusariosis in patients with hematologic malignancies. PLoS ONE. 2018;13:e0196426.CrossRefGoogle Scholar
  11. 11.
    Schell WA. New aspects of emerging fungal pathogens: a multifaceted challenge. Clin Lab Med. 1995;15:365–87.CrossRefGoogle Scholar
  12. 12.
    Liu K, Howell DN, Perfect JR, Schell WA. Morphologic criteria for the preliminary identification of Fusarium, Paecilomyces, and Acremonium species by histopathology. Am J Clin Pathol. 1998;109:45–54.CrossRefGoogle Scholar
  13. 13.
    Thomas PA, Nelaon Jesudasan CA. Adventitious sporulation in Fusarium keratitis. Graefes Arch Clin Exp Ophthalmol. 2011;249:1429–31.CrossRefGoogle Scholar
  14. 14.
    Khan ZU, Ahmad S, Theyyathel AM. Detection of Aspergillus fumigatus-specific DNA, (1-3)-β -d-glucan and galactomannan in serum and bronchoalveolar lavage specimens of experimentally infected rats. Mycoses. 2008;51:129–35.CrossRefGoogle Scholar
  15. 15.
    Khan ZU, Ahmad S, Mokaddas E, Chandy R, Cano J, Guarro J. Actinomucor elegans var. kuwaitiensis isolated from the wound of a diabetic patient. Antonie van Leeuwoenhoek. 2008;94:343–52.CrossRefGoogle Scholar
  16. 16.
    Khan Z, Ahmad S, Al-Ghimlas F, Al-Mutairi S, Joseph L, Chandy R, Sutton DA, Guarro J. Purpureocillium lilacinum as a cause of cavitary pulmonary disease: a new clinical presentation and observations on atypical morphologic characteristics of the isolate. J Clin Microbiol. 2012;50:1800–4.CrossRefGoogle Scholar
  17. 17.
    Wolf FT. Nutrition and metabolism of the tobacco wilt Fusarium. Bull Torrey Bot Club. 1955;82:343–54.CrossRefGoogle Scholar
  18. 18.
    Kidd GH, Wolf FT. Dimorphism in a pathogenic Fusarium. Mycologia. 1973;65:1371–5.CrossRefGoogle Scholar
  19. 19.
    Lockwood MB, Crescencio JCR. Adventitious sporulation in Fusarium: the yeast that were not. ID Cases. 2016;3:5–7.PubMedGoogle Scholar
  20. 20.
    Muhammed M, Anagnostou T, Desalermos A, Kourkoumpetis TK, Carneiro HA, Glavis-Bloom J, Coleman JJ, Mylonakis E. Fusarium infection: report of 26 cases and review of 97 cases from the literature. Medicine. 2013;92:305–16.PubMedPubMedCentralGoogle Scholar
  21. 21.
    Nucci M, Carlesse F, Cappellano P, Varon AG, Seber A, Garnica M, Nouér SA, Colombo AL. Early diagnosis of invasive fusariosis with Aspergillus serum galactomannan testing. PLoS ONE. 2014;9:e87784.CrossRefGoogle Scholar
  22. 22.
    Short DPG, O’Donnell K, Zhang N, Juba JH, Geiser DM. Widespread occurence of human pathgenic types of the fungus Fusarium detected in plumbing drains. J Clin Microbiol. 2011;49:4264–72.CrossRefGoogle Scholar
  23. 23.
    Mehl HL, Epstein L. Sewage and community shower drains are environmental reservoirs of Fusarium solani species complex group 1, a human and plant pathogen. Environ Microbiol. 2008;2008(10):219–27.Google Scholar
  24. 24.
    Khor WB, Aung T, Saw SM, Wong TY, Tambyah PA, Tan AL, Beuerman R, Lim L, Chan WK, Heng WJ, Lim J, Loh RS, Lee SB, Tan DT. An outbreak of Fusarium keratitis associated with contact lens wear in Singapore. J Am Med Assoc. 2006;295:2867–73.CrossRefGoogle Scholar
  25. 25.
    Chang DC, Grant GB, O’Donnell K, Wannemuehler KA, Noble-Wang J, Rao CY, Jacobson LM, Crowell CS, Sneed RS, Lewis FM, Schaffzin JK, Kainer MA, Genese CA, Alfonso EC, Jones DB, Srinivasan A, Fridkin SK, Park BJ. Fusarium keratitis investigation team multistate outbreak of Fusarium keratitis associated with use of a contact lens solution. J Am Med Assoc. 2006;296:953–63.CrossRefGoogle Scholar
  26. 26.
    Ahear DG, Zhang S, Ward MA, Simmons RB, Stulting RD. Hyphal penetration of worn hydrogel contact lenses by Fusarium. Cornea. 2009;28:914–7.CrossRefGoogle Scholar
  27. 27.
    van Diepeningen AD, Al-Hatmi AMS, Brankovics B, de Hoog GS. Taxonomy and clinical spectra of Fusarium Species: Where do we stand in 2014? Curr Clin Microbiol Rep. 2014;1:10–8.CrossRefGoogle Scholar
  28. 28.
    Nir-Paz R, Strahilevitz J, Shapiro M, Keller N, Goldschmied-Reouven A, Yarden O, Block C, Polacheck I. Clinical and epidemiological aspects of infections caused by Fusarium species: a collaborative study from Israel. J Clin Microbiol. 2004;42:3456–61.CrossRefGoogle Scholar
  29. 29.
    Taj-Aldeen SJ, Salah H, Al-Hatmi AM, Hamed M, Theelen B, van Diepeningen AD, Boekhout T, Lass-Flörl C. In vitro resistance of clinical Fusarium species to amphotericin B and voriconazole using the EUCAST antifungal susceptibility method. Diagn Microbiol Infect Dis. 2016;85:438–43.CrossRefGoogle Scholar
  30. 30.
    Al-Hatmi AMS, Curfs-Breuker I, de Hoog GS, Meis JF, Verweij PE. Antifungal susceptibility testing of Fusarium: a practical approach. J Fungi. 2017;3(2):E19.CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Microbiology, Faculty of MedicineKuwait UniversitySafatKuwait

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