, Volume 184, Issue 1, pp 45–51 | Cite as

Genetic Diversity of the Cryptococcus gattii Species Complex in Mato Grosso State, Brazil

  • Fernanda Harumi MaruyamaEmail author
  • Daphine Ariadne Jesus de Paula
  • Isabela de Godoy Menezes
  • Olívia Cometti Favalessa
  • Rosane Christine Hahn
  • Arleana do Bom Parto Ferreira de Almeida
  • Valéria Régia Franco Sousa
  • Luciano Nakazato
  • Valéria Dutra
Short Communication


Cryptococcosis is caused by fungi of the genus Cryptococcus. Owing to its importance, this study aimed to analyze the genetic diversity of C. gattii isolates from animals, humans, and the environment in Mato Grosso State (MT), Brazil, during November 2010–December 2017. All isolates of the C. gattii species complex were subjected to molecular genotyping via Restriction Fragment Length Polymorphism (PCR–RFLP) and Multi-locus Sequence Typing (MLST). PCR–RFLP analysis revealed that 21 isolates presented the genotype VGII, which is considered the most common and virulent genotype globally among. MLST analysis revealed the presence of 14 sequence types (STs), of which 5 are considered new genotypes. Clonal Complex (CC) CC182 (n = 5; 23,80%) and CC309 (n = 3; 14,28%) were the most frequent. CC distribution in relation to origin revealed that three CCs were found in animals with a predominance of CC182 (66,66%), while nine were found in humans, and two CCs were found in the environment. Extensive genetic variability was observed among the isolates in the State of Mato Grosso. STs belonging to the already described clonal complexes (CC) indicate the global expansion and adaptation of isolates in several other countries. Therefore, detection of clonal complexes and STs already described in other regions and the occurrence of new STs in the present study help further the current understanding of the geographic dispersion and genetic origin of the C. gattii species complex.


Cryptococcus gattii Multi-locus sequence typing Genotyping Cryptococcosis 



Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical Approval

This study was certificated of the Animal and Human Ethics Committee No. 888/CEP-HUJM/2012.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Fernanda Harumi Maruyama
    • 1
    Email author
  • Daphine Ariadne Jesus de Paula
    • 1
  • Isabela de Godoy Menezes
    • 1
  • Olívia Cometti Favalessa
    • 2
  • Rosane Christine Hahn
    • 2
  • Arleana do Bom Parto Ferreira de Almeida
    • 1
  • Valéria Régia Franco Sousa
    • 1
  • Luciano Nakazato
    • 1
  • Valéria Dutra
    • 1
  1. 1.Postgraduate Course in Veterinary Sciences, Department of Veterinary SciencesFederal University of Mato GrossoCuiabáBrazil
  2. 2.Postgraduate Course in Health Sciences, Department of Molecular BiologyFederal University of Mato GrossoCuiabáBrazil

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