Up-Regulation of Antimicrobial Peptides Gallerimycin and Galiomicin in Galleria mellonella Infected with Candida Yeasts Displaying Different Virulence Traits
- 64 Downloads
Galleria mellonella has been described as a cheap and an easy-to-reproduce model for the study of fungal infections. We hypothesized that yeasts with higher virulence potential decrease survival and significantly trigger an immune response in G. mellonella through the regulation of innate immunity-related genes encoding antimicrobial peptides (AMPs) such as gallerimycin and galiomicin. Candida albicans SC5314 and Candida dubliniensis CBS 7987, selected because of their different virulence potential, were used for a killing assay followed by the determination of gene expression using qPCR. In vivo results confirmed a significantly (p = 0.0321) lower pathogenicity for C. dubliniensis than for C. albicans. Accordingly, the induction of C. dubliniensis AMPs was lower at all the selected time points post-infection (1 h, 24 h, 48 h). Moreover, we observed an extremely high regulation of the galiomicin gene compared to the gallerimycin one, suggesting a different role of the tested AMPs in protecting G. mellonella from candidiasis.
KeywordsG. mellonella Gallerimycin Galiomicin Gene expression C. albicans C. dubliniensis
This work was supported by the Slovak Research and Development Agency under Contract No. [APVV-15-0347 to H.B.] and by the Grant VEGA [1/0628/15 to H.B.] supported by the Ministry of Education, Science, Research and Sport of the Slovak Republic. The authors wish to thank Dr. Stanislava Dižová for help with the qPCR technique.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 4.Rossoni RD, Fuchs BB, de Barros PP, Velloso MDS, Jorge AOC, Junquiera JC, et al. Lactobacillus paracasei modulates the immune system of Galleria mellonella and protects against Candida albicans infection. PLoS ONE. 2017;12(3):e0173332. https://doi.org/10.1371/journal.pone.0173332.CrossRefPubMedPubMedCentralGoogle Scholar
- 5.Sheehan G, Kavanagh K. Analysis of the early cellular and humoral responses of Galleria mellonella larvae to infection by Candida albicans. Virulence. 2017;5:1–10.Google Scholar
- 13.Moran GP, Coleman DC, Sullivan DJ. Candida albicans versus Candida dubliniensis: why is C. albicans more pathogenic? Int J Microbiol. 2012; Article ID 205921, 7 pp.Google Scholar
- 26.Chupáčová J, Borghi E, Morace G, Los A, Bujdáková H. Anti-biofilm activity of antibody directed against surface antigen complement receptor 3-related protein-comparison of Candida albicans and Candida dubliniensis. Pathog Dis. 2018;76(1):127. https://doi.org/10.1093/femspd/ftx127.CrossRefGoogle Scholar