Molecular characterization of the Krüppel-homolog 1 and its role in ovarian development in Sogatella furcifera (Hemiptera: Delphacidae)

  • Kui Hu
  • Ping Tian
  • Lu Yang
  • Yan Tang
  • Lin Qiu
  • Hualiang He
  • Wenbing Ding
  • Youzhi LiEmail author
Original Article


Juvenile hormone (JH) plays a pivotal role in insect reproduction. The Krüppel-homolog 1 (Kr-h1) is a JH-inducible zinc finger transcription factor that has also been found to play a role in insect reproduction, however, its function varies across species. In this study, we cloned SfKr-h1 from Sogatella furcifera and investigated its role in ovarian development. The open reading frame of SfKr-h1 is 1 800 bp encoding 599 amino acids. The putative amino acid sequence of SfKr-h1 contains eight putative C2H2-type zinc finger domains and is highly homologous with the Kr-h1s of other hemipteran species. Expression of SfKr-h1 peaked 96 h after adult emergence and was highest in the ovary. RNA interference (RNAi) knockdown of SfKr-h1 substantially reduced the transcription of SfVg, and arrested ovarian development. These results suggest that SfKr-h1 is critical for normal ovarian development in S. furcifera.


Sogatella furcifera Krüppel-homolog 1 Reproduction Juvenile hormone RNA interference 



This work was funded by National Natural Science Foundation of China (31572005) and Double First-class Construction Project of Hunan Agricultural University (SYL2019029).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant ProtectionHunan Agricultural UniversityChangshaChina
  2. 2.National Research Center of Engineering & Technology for Utilization of Botanical Functional IngredientsHunan Agricultural UniversityChangshaChina
  3. 3.Hunan Provincial Engineering & Technology Research Center for Biopesticide and Formulation ProcessingHunan Agricultural UniversityChangshaChina

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