Strong genetic differentiation of the clam Meretrix lamarckii in the China Sea revealed by mitochondrial DNA marker
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The hard clam Meretrix lamarckii is ecologically and economically important in the coastal regions of China. In this study, we evaluated the genetic diversity and population structure of six M. lamarckii populations in the East China Sea and the South China Sea using mitochondrial cytochrome c oxidase subunit 1 (COI) and cytochrome b (Cytb) genes. We obtained 582 bp of partly sequences comprising 28 novel haplotypes of COI gene from 138 specimens and 1168 bp of partly sequences comprising 22 novel haplotypes of Cytb gene from 125 specimens. The haplotype diversity of COI and Cytb genes ranged from 0.606 to 0.862 and 0.562 to 0.863, respectively. The nucleotide diversity ranged from 0.0015 to 0.0038 in COI gene and ranged from 0.0007 to 0.0032 in Cytb gene. Thus, there is moderate-level genetic diversity in M. lamarckii in the China Sea. The F-statistics showed that the Zhoushan (ZS) and Xiangshan (XS) populations were significantly (P < 0.01) differed from the populations of Wenzhou (WZ), Zhangpu (ZP), Shantou (ST), and Zhanjiang (ZJ) in both COI and Cytb genes. Both haplotypes network and plot of STRUCTURE analysis suggested obviously genetic divergence between East China Sea and South China Sea regions. Knowledge on genetic variation and population structure of M. lamarckii populations along the Southeast China Sea obtained from this study will support the aquaculture management and conservation of M. lamarckii in China.
KeywordsMeretrix lamarckii Genetic diversity COI Cytb
Y Y and B G designed the work, analyzed the data, acquisition the data, interpreted the results, wrote the manuscript, J F, Z F and Y G collected samples and prepared genomic DNA, performed experimental works, and check the data, J L and Z L designed the work, analyzed the data, wrote the manuscript, and provided funds for this study. All authors have given final approval for the publication of the article.
This work was financially supported by the Natural Science Foundation of Zhejiang Province (Grant No.: LQ18D060004), Scientific Research Startup Foundation of Zhejiang Ocean University (Funding No.: 12245090318) and the Project of Bureau of Science and Technology of Zhoushan (Funding No.: 2019F12004).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
“All applicable international, national, and/or institutional guidelines for the care and use of animals were followed by the authors.”
- 9.Felsenstein J (1989) PHYLIP—Phylogeny inference package (version 32). Cladistics 5:164–166Google Scholar
- 15.Hedgecock D (1994) Temporal and spatial genetic structure of marine animal populations in the California Current. Cal Coop Ocean Fish 35:73–81Google Scholar
- 18.Li HJ, Zhang JJ, Yuan XT et al (2016) Genetic diversity and differentiation of seven geographical populations of hard clam (Meretrix meretrix) assessed by COI and microsatellite markers (in Chinese with English abstract). Acta Ecol Sin 36(2):499–507Google Scholar
- 19.Jackson GA (1986) Interaction of physical and biological processes in the settlement of planktonic larvae. Bull Mar Sci 39:202–212Google Scholar
- 23.Ma C (2009) The effects of the Kuroshio on the main current systems in the East China Sea. PhD thesis, Ocean University of China, Qingdao, 52–66Google Scholar
- 25.Pritchard JK, Wen X, Falush D (2009) Documentation for STRUCTURE software: version 2.3. Comment J Pediatr Surg 41:289–293Google Scholar
- 27.Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425Google Scholar
- 28.Shao YQ, Zhang JM, Fang J et al (2017) Reproductive cycle and early development of Meretrix lamarkii (Veneroida: Veneridae) under artificial conditions (in Chinese with English abstract). J Fish Sci China 24:82–90Google Scholar
- 31.Teng SS, Fang J, Jin WH et al (2015) Analysis of genetic diversity in four different Meretrix lamarckii geographical populations using microsatellite markers (in Chinese with English abstract). J Fish Sci ChinaGoogle Scholar
- 37.Yuan WB, Li CB, Jiao HF et al (2017) Genetic resources assessment of mussel Mytilus coruscus based on COI gene in Yushan Island (in Chinese with English abstract). Mar Sci 41:107–112Google Scholar
- 38.Zhang SP, Wang HX, Xu FS (2012) Taxonomic study on Meretrix (Bivalvia, veneridae) from China seas (in Chinese with English abstract). Acta Zootaxonomica Sin 37:473–479Google Scholar
- 39.Zhang JL, Wang QH, Deng YW (2014) Effects of morphological traits on the weight of Meretrix lamarchii. Prog Fish Sci 35:110–113Google Scholar
- 41.Zhuang QQ (2001) Fauna Sinica: Invertebrata: Mollusca: Bivalvia: Veneridae (in Chinese with English abstract). Science Press, BeijingGoogle Scholar